Syllabus
Genome 371: Introduction to Genetics, Autumn 2002
| Date | Topic | Reading Assignment | Problems |
| Monday, Sep 30 | 1. Introduction to Genetics: DNA, the central dogma, protein structure, ORFs, the code, yeast genes | 1-6, 150-157, 222-237, 249-251 | Ch 5: 7, 8; Ch 7: 1, 9, 11, 16; Ch 18: 2 |
| Tuesday, Oct 1 | QS 1. From DNA sequence to protein function -- finding genes in DNA sequence | . | . |
| Wednesday, Oct 2 | 2. Mendelian Segregation: variation in protein function, dominance/recessiveness, probability, testcrosses, pedigrees | 10-21 | Ch 1: 1, 6, 7, 8, 9, 13, 23, 25, 26, 27 |
| Friday, Oct 4 | 3. Independent Assortment: dihybrids, chi-squared analysis | 21-31, 111 | Ch 1: 4, 16, 17, 18, 20, 21, 22 |
| Monday, Oct 7 | 4. Genotype to Phenotype: incomplete dominance, codominance, multiple alleles, pleitropy, lethality | 38-49, 201-212 | Ch 2: 1, 3, 4, 7, 8, 9, 12, 13,16, 17, 20, 21, 26 |
| Tuesday, Oct 8 | QS 2. Cat phenotypes
Pretest |
. | . |
| Wednesday, Oct 9 | 5. Genetic Interactions: the complementation test, introduction to epistasis and complex traits | 49-63, 191, 194, 195 | Ch 2: 11, 15, 18, 23; Ch 6: 12, 18, 19 |
| Friday, Oct 11 | 6. Chromosomes: ploidy, homologues, sex, mitosis, meiosis | 70-91 | Ch 3: 1, 5, 7, 10, 13, 14 |
| Monday, Oct 14 | 7. Chromosome Theory of Inheritance; sex linkage, aneuploidy | 91-98, 443-451 | Ch 3: 16, 17, 18, 20, 22, 25, 27 |
| Tuesday, Oct 15 | QS 3. Yeast genetics | . | . |
| Wednesday, Oct 16 | 8. Review for Exam I | . | Cats for Practice Exam 1 |
| Friday, Oct 18 | Exam I: 2:30- 4:20
Lectures 1 - 8, QS 1 - 3 |
. | Exam Keys and Regrade Policy |
| Monday, Oct 21 | 9. Linkage vs. Independent Assortment; Recombination; chi-squared analysis | 105-118 | Ch 4: 2, 6, 8, 10, 11, 12, 13 |
| Tuesday, Oct 22 | QS 4. Yeast tetrads | 125-135 | . |
| Wednesday, Oct 23 | 10. Chromosome Mapping: 2- and 3- point crosses | 118-125 | Ch 4: 1, 14, 15, 17, 18, 22, 23, 25, 26 |
| Friday, Oct 25 | 11. Molecular basis for recombination | 164-165, 168-174, 83-87, 212-214 | Ch 5: 21, 22, 23, 24; Ch 6: 16, 25 |
| Monday, Oct 28 | 12. Mutation and DNA repair | 179-193 | Ch 6: 1, 3, 10, 26 |
| Tuesday, Oct 29 | QS 5. Fly Cross: sex-linked and autosomal traits, mapping | 702-710 | . |
| Wednesday, Oct 30 | 13. Transposons and chromosome rearrangements | 419-423, 426-443 | Ch 12: 1, 4, 7, 8, 13, 16, 19, 20 |
| Friday, Nov 1 | 14. Review for Exam II | . | . |
| Monday, Nov 4 | Exam II: 2:30- 4:20
Lectures 9 - 14, QS 4 - 5 |
. | Exam Keys and Regrade Policy |
| Tuesday, Nov 5 | QS 6. Identification of mutant flies | . | . |
| Wednesday, Nov 6 | 15. Mutagenesis in yeast: the red phenotype; reversion and suppression | 638-643, 208-209 | Ch 6: 11, 13, 17, 20, 22 |
| Friday, Nov 8 | 16. Mutant hunts in yeast: centromeres and kinetochores | 390-404, 626-636 | Ch 18: 6, 10, 12 |
| Monday, Nov 11 | Veteran's Day Holiday | . | . |
| Tuesday, Nov 12 | QS 7. Analysis of yeast MCM mutants | . | . |
| Wednesday, Nov 13 | 17. DNA methods: restriction enzymes; cloning; genomic and cDNA libraries | 262-277, 166-167 | Ch 8: 1, 3 6, 9, 17, 20 |
| Friday, Nov 15 | 18. DNA methods continued: hybridization, probes, PCR, sequencing | 157-164, 277-295 | Ch 8: 22, 24, 25; Ch 12: 15 |
| Monday, Nov 18 | 19. Molecular detection of genotype: repeats, deletions, insertions, RFLPs | 308-318, 322-324, 326-334,188-189 | Ch 9: 1, 4, 5, 6, 21; Ch 12: 5, 22, 25 |
| Tuesday, Nov 19 | QS 8. Isolation of DNA from mutant flies | . | . |
| Wednesday, Nov 20 | 20. DNA markers; mapping human disease genes | 358-359, 371-378, 124-125 | Ch 9: 7, 8, 9, 14, 15 |
| Friday, Nov 22 | 21. Review for Exam III | . | . |
| Monday, Nov 25 | Exam III: 2:30- 4:20
Lectures 15 - 21, QS 6 - 8 |
. | Exam Keys and Regrade Policy |
| Tuesday, Nov 26 | QS 9. Gel elctrophoresis and DNA sequencing reactions | . | . |
| Wednesday, Nov 27 | 22. Analysis of whole genomes: the sequence of the human genome | 341-371 | Ch 10: 1, 3, 5, 6, 8, 9 |
| Friday, Nov 29 | Thanksgiving Holiday | . | . |
| Monday, Dec 2 | 23. The Drosophila genome: comparison of yeast, fly and human genomes/genes; Introduction to fly mutant hunts | 702-714, 208-209, 821 | Ch 10: 19, 20, 22; Ch 21: 1 |
| Tuesday, Dec 3 | QS 10. Sequencing results and BLAST search on fly mutant DNA | . | . |
| Wednesday, Dec 4 | 24. Fly mutant hunts | 626-637, 714-725 | Ch 6: 7, 8; Ch 21: 3 |
| Friday, Dec 6 | 25. Analyzing fly mutants: phenotype -> biological function; mapping; complementation tests; epistasis; molecular function -> phenotype | . | Ch 6: 23; Ch 21: 2, 9, 10, 12, 13, 14 |
| Monday, Dec 9 | 26. Modifier Screens; application to human biology and complex traits | 814-820 | Ch 10: 25 |
| Tuesday, Dec 10 | QS 11. Fly pathway analsyis | . | . |
| Wednesday, Dec 11 | 27. Fly mutants summary; Review for Final Exam | . | . |
| Monday, Dec 16 | FINAL EXAM: 2:30 - 4:20
Lectures 1-28 and QS 1-11, with an emphasis on Lectures 22-28 and QS 9-11 |
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