Education and Training:

M.D., Lanzhou Medical School, China (1984)

Resident, Internal Medicine, Tianshuei Medical School, China (1984-85)

Resident, General Surgery, Research Fellow in Neurobiology, Tianshuei Medical School (1985-86)

Resident with research track, Neurosurgery and Neurobiology, Tianjin Medical School, China (1986-89)

M.S., Neurobiology, Tianjin Neurology Institute, Tianjin Medical College (1989)

Neurosurgery Clinical Fellowship, Department of Neurosurgery, University of Birmingham, United Kingdom (1990-93)

Ph.D., Neurobiology, University of  Birmingham/ Wolverhampton, United Kingdom (1993)

Postdoctoral Fellow, Gladstone Institute of Cardiovascular Disease, Cardiovascular Institute, University of California, San Francisco (1993-94)

CURRENT RESEARCH INTERESTS

Thrombotic thrombocytopenic pupura, ADAMTS-13 biology, and platelet functions under fluid shear stress

RESEARCH DESCRIPTION

Research in our group has been concentrated on platelet functions under high fluid shear stress. Our aim is to understand how platelets interact with subendothelium, with each other, and with other blood cells under flow condition. We are particularly interested in dissecting signal pathways that are initiated by the interaction of platelet glycoprotein Ib-IX-V complex and von Willebrand factor.

Our research also focuses on defining mechanisms through which the endothelium-derived ultra-large von Willebrand factor multimers are cleaved by the ADAMTS-13 metalloprotease in vivo and what roles of this proteolysis process may play in the development of thrombosis.

Such efforts will allow us to identify agents that selectively block pathophysiological thrombosis without affecting physioligcal hemostatic function of platelets.

Dong J.F., Moake J.L., Nolasco L., Bernardo A., Arceneaux W., Shrimpton C.N., Schade A.J., McIntire L.V., Fujikawa K., López J.A.  (2002) ADAMTS-13 rapidly cleaves newly secreted ultra-large von Willebrand factor multimers on the endothelial surface under flowing conditions. Blood 100(12):4033-4039.

Dong J.F., Moake J.L., Bernardo A., Fujikawa K., Ball C., Nolasco L., López J.A.., Cruz M.A. (2003) ADAMTS-13 metalloprotease interacts with the endothelial cell-derived ultra-large von Willebrand factor.  J Biol. Chem. 278(32):29633-29639.

Padilla A., Moake J.L., Bernardo A., Ball C., Wang Y., Arya M., Nolasco L., Turner N., Berndt M.C., Anvari B., López J.A.., Dong J.F. (2004)  P-selectin anchors newly released ultra-large von Willebrand factor multimers to the endothelial cell surface. Blood 103(6):2150-2156.

Bernardo A., Ball C., Nolasco L., Moake J.F., Dong J.F.  (2004) Effects of inflammatory cytokines on the release and cleavage of the endothelial cell-derived ultra-large von Willebrand factor multimers under flow. Blood 104(1):100-106.

Bernardo A., Ball C., Nolasco L., Choi H., Moake J.L., Dong J.F. (2005) Platelets adhered to endothelial cell-bound ultra-large von Willebrand factor strings support leukocyte tethering and rolling under high shear stress.  J Thromb Haemost 3(3):562-570.

Tao Z., Wang Y., Choi H., Bernardo A., Nishio K., Sadler J.E., López J.A., Dong J.F. (2005) Cleavage of ultra-large multimers of von Willebrand factor by C-terminal-truncated mutants of ADAMTS-13 under flow. Blood 106(1):141-143.

Li Liu, Tao Z, Ruan, Moake J, Dong J.F. (2005) Platelet-derived ADAMTS-13. J Thromb. Haemost. 3(11):2536-2544

Tao Z, Peng Y Cal S, Moake J, Lopez JA, Dong J.F (2005) CUB domain anchors ADAMTS-13 to ULVWF strings under flow conditions. Blood  106(13):4139-4145

Tao Z, Anthony K, Peng Y, Choi H, Nolasco L, Rice L, Moake JL, Dong JF. (2006) Novel ADAMTS-13 mutations in an adult with delayed onset thrombotic thrombocytopenic purpura. J Thromb Hemost. 4:1931-1935

Choi H, Aboulfatova K, Pownall HJ, Cook R, Dong JF. (2008) shear-induced disulfide bond formation regulates adhesion activity of von Willebrand factor^. J Biol. Chem. 282:35604-35611 

Li Y,  Choi H, Zhou Z, Nolasco L,  Pownall HJ,  Voorberg J,  Moake JL, Dong J.F. (2008) Covalent regulation of ULVWF string formation and elongation on endothelial cells under flow conditions. J Thromb. Hemost 6(7):1135-1143

Wu T, Lin J, Cruz MA, Dong J.F. and Zhu C. (2009) Force-induced Cleavage of Single VWF A1A2A3-tridomains by ADAMTS-13. Blood ( co-corresponding author) 115:370-378

Saunders J, Nambi V, Kimball KT, Virani S, Yang E, Bergeron A, Athamneh H, Kumar A,  Morrisett JD, Lumsden A,  Chen J, Lin P, Bechara C, Ballantyne CM, Dong J.F. (2010) Variability and Persistence of Aspirin Response to lower extremity peripheral artery disease patients. J. Vasc. Surg. 53(3);668-675

Yeh HC, Zhou X, Choi H, Tekeoglu S, May W, Turner N, Scheiflinger F, Moake JL, Dong JF. (2010) Disulfide bond reduction of von Willebrand factor by ADAMTS-13. J Thromb. Hemost. 8:2278-2288

Campos M, Sun W, Yu F, Barbalic M, Tang T, Chambless LE, Ballantyne CM, Folsom AR, Boerwinkle E, Dong J.F. (2011) Genetic Determinants of Plasma von Willebrand Factor Antigen Levels: A Target Gene SNP and Haplotype Analysis of ARIC Cohort. Blood (Epub ahead of print)