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Case 2: Features of Genotypic and Phenotypic Resistance Assays

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B The M184V mutation notation refers to the replacement of the amino acid methionine (M) by valine (V) at amino position 184.

This answer is correct. The HIV genotype is typically performed using the reverse transcriptase polymerase chain reaction (PCR) to amplify HIV DNA from HIV RNA, followed by sequencing of the HIV DNA gene regions of interest. This process can determine specific HIV nucleotide mutations that differ from wild-type HIV. These results are then used to infer amino acid changes that would result from specific mutations. The genotype resistance reports use a shorthand convention that lists the wild type amino acid, followed by the position of this amino acid, followed by the amino acid that has replaced the wild type amino acid (at the position listed).


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