Glassware Washing and Sterilization
We have one large steam sterilizer dedicated to the sterilization of biohazardous waste with a 60 minute cycle at 121° C. We are able to process 9 loads of biohazardous waste at a time from our processing cart, which has 3 shelves and a length of 4’ x height of 2’ x depth of 1’8” per shelf. 3:30 pm is when the last load of the day will be run.
We adhere to the following Standard Operating Procedure, which must be followed before bringing your biohazardous waste for processing:
- Collect solid biohazardous waste in an autoclavable biohazard bag no larger than 19 x 23, attach autoclave tape, transport in a leak-proof secondary container, and load your materials into plastic trays on the processing cart in T-276, filling up the lower shelves of the processing cart first. Each filled plastic tray is considered one load. Please see the EH&S Biohazardous Waste guidelines on how to package and transport your biohazardous waste.
- All sharps waste must be placed in red sharps containers marked with the biohazardous symbol no larger than 12 x 12 x 12, attach autoclave tape, and labeled with the Principal Investigator's name and the room number. Make sure the autoclave tape is not placed over the vent holes in the top of the sharps container, as these are used to allow steam to reach the contents and to allow for expansion and contraction of the container during autoclaving. Use appropriate size sharps containers and do not fill more than two-thirds full before loading onto the processing cart in T-276.
- Broken glassware contaminated with biohazardous material must be placed in a sharps container no larger than 12 x 12 x 12 for proper disposal, attach autoclave tape and labeled with the Principal Investigator's name and the room number before loading onto the processing cart in T-276.
- Plastic pipettes and pipette tips contaminated with biohazardous material should be placed in a container that is easily autoclaved and does not allow the tips to puncture it. One possible solution is to use a pipette box no larger than 12 x 12 x 12. Attach autoclave tape to the container and transport in a leak-proof secondary container before loading onto the processing cart in T-276.
- Refer to EH&S Biohazardous Waste for more information about identification, decontamination, segregation, packaging, transporting, and disposal.
- Refer to EH&S Sharps, Laboratory Glass, and Plasticware Waste for more information about sharps waste.
- The Magnuson Health Sciences Flow Chart (pdf) summarizes the proper packaging, treatment and disposal of biological and biomedical waste for locations within Magnuson Health Sciences, W.H. Foege Genome Sciences, and W.H. Foege Bioengineering.
We have two autoclaves dedicated to the steam sterilization of reagents and laboratory supplies, such as pipette tips, caps, mixed glassware, and instruments. Please drop off supplies in T-276, loading your supplies onto a small autoclave cart. Special exposure times and temperatures can be requested. Once autoclaved these can be picked up from T-286.
For those laboratories doing cell culture work, we have an oven available to do high-heat dry sterilization. Please drop off materials in T-276 to be processed for 3 hours at 195 C. Once autoclaved these can be picked up from T-286.
For any of the three services above you will need to fill out our Sign-In Sheet each time, providing the following information:
- Date of Service
- Laboratory Name
- Room Number of Laboratory
- Phone Number
- Budget Number