Samuel I. Miller, MD
Professor of Genome Sciences, Medicine, Immunology and Microbiology
Email: click here
Phone: (206) 616-5110
The Miller laboratory is focused on defining the molecular basis of bacterial pathogenesis and interactions with eukaryotic cells. The laboratory has a particular interest in bacterial interactions with innate immunity. This work involves the use of animal and tissue culture (mice, macrophages, epithelial cells) models of infection using Salmonella, Pseudomonas, and Yersinia. Research interests include Salmonellae-induced typhoid fever and gastroenteritis, the chronic Pseudomonas airway disease of cystic fibrosis patients, and Gram-negative organisms important to biodefense, including Francisella tularensis, the plague bacillis Yersinia pestis, and Burkholderia spp. The lab is organized into research groups focusing on the study of: (1) The effect of bacterial type III effector proteins on mammalian cells; (2) the assembly and regulation of the type III secretion system of Salmonella typhimurium, which translocates proteins into mammalian cells on contact; (3) the environmental remodeling of the Gram-negative bacterial surface that occurs when bacteria infect host tissues; (4) the characterization of the phenotypic adaptation of Pseudomonas aeruginosa to the unique environmental niche of the cystic fibrosis airway; (5) analysis of bacterial genes and proteins using bioinformatics; (6) development of new antimicrobial compounds inhibiting bacterial secretion, cell signaling, and outer membrane permeability; (7) understanding sensing and signaling by bacterial receptors, particularily the PhoQ protein of Salmonella; and (8) human susceptibility to bacterial infection and diversity of human inflammatory responses.
Rakeman JL, Bonifield HR, Miller SI. A HilA-independent pathway to Salmonella typhimurium invasion gene transcription. J Bacteriol 181(10):3096-104, 1999.
Miao EA, Miller SI. Bacteriophages in the evolution of pathogen-host interactions. Proc Natl Acad Sci USA 96(17):9452-4, 1999.
Tsolis RM, Townsend SM, Miao EA, Miller SI, Ficht TA, Adams LG, Baumler AJ. Identification of a putative Salmonella enterica serotype typhimurium host range factor with homology to IpaH and YopM by signature-tagged mutagenesis. Infect Immun 67(12):6385-93, 1999.
Guina T, Yi EC, Wang H, Hackett M, Miller SI. A PhoP-regulated outer membrane protease of Salmonella enterica serovar typhimurium promotes resistance to alpha-helical antimicrobial peptides. J Bacteriol 182(14):4077-86, 2000.
Scherer CA, Cooper E, Miller SI. The salmonella type III secretion translocon protein SspC is inserted into the epithelial cell plasma membrane upon infection. Mol Microbiol 37(5):1133-45, 2000.