User Group Meeting, 2011 Nov 22

Fun stuff

• Imaging Facility website: layout and equipment
• EM: SEM, TEM (performance and limitation)
  • examples of SEM images
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      filter paper at 1000×

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      a grating replica with a "d" spacing of 0.4629 µm at 10k×

  • resolution of our TEM
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      negative staining of T4 phage at 130k×

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      negative staining of T4 phage at 130k×

• Confocal: comparison, performance of the SP5 63× oil immersion, 1.4 NA
  • resolution
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      xz profile plot of a mirror, FWHM is 348 nm

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      gallery view of an xzy series of a 175 nm fluorescent microsphere, FWHM is 472 nm (z), 222 nm (x), and 192 (y)

  • color registration
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      xy profile plot of a 4 µm tetraspeck fluorescent microsphere, deviation is about 500 nm

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      xz profile plot of a 4 µm tetraspeck fluorescent microsphere, deviation is >2 µm

  • applications: tile-scan, mark and find, bleaching/photoactivation, FRAP and FRET, linear unmixing
  • optimization: limitation, aberration
• postprocessing of images: general guidelines about image manipulation in The NIH Catalyst
• software packages: ImageJ/Fiji, Bioimagexd, FARSight

Policy

current policy

Open discussion/suggestion

There were some questions about how to set up an appointment and a suggestion to require users to update the schedule when finish early or cannot make the appointment. Our policy has been updated to included this requirement.