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| parameter | Olympus IX81 with DSU | Leica SP5 II |
|---|---|---|
| general operation | software driven, spinning disk with slit formation | software driven, single variable pinhole |
| setup | none | none |
| available excitation | 4 bands: 387, 485, 560, 650 nm from a Xenon plasma lamp | 7 laser lines: 405, 458, 476, 488, 514, 561, 633 nm |
| 2-photon | no | no |
| excitation power control | continuous | continuous |
| microscope stand | inverted, fully motorized | inverted, fully motorized |
| DIC | excellent | excellent |
| max. image size (pixel) | 512×512 | 8192×8192 |
| max. frame rate (fps, pixel) | ~30, 512×512 | 5 (28 with resonant scanner), 512×512 |
| detector | EM-CCD | 5 PMT |
| max # of simultaneous scans | 1 confocal or transmitted light | 4 spectral confocal + 1 transmitted light |
| max # of sequential scans | 5 | 16 |
| live imaging and time series | good | good |
| overall image quality (Click to show/hide details) |
good | excellent |
| confocality | good | excellent |
|---|---|---|
| illumination e.g., evenness, centrality |
good | excellent |
| detector sensitivity | excellant | good |
| channel co-registration | good | excellent |