Protocols

NTS Buffer/DAPI Stain

To 500ml sterile water add:

8.5 g NaCl (final Conc.=1mM)

1.2 g Tris Base ( final conc.=10mM)

Sigma #T1503

Adjust pH to 7.8 with HCl

Add:

0.111 g CaCl₂ ( final conc.=1mM)

Fisher #C70-500

0.123 g MgSO₄*7H₂O (final conc.=0.5mM)

0.5 g BSA

Sigma# A6003

1 ml NP-40

Sigma# N-3516

Add sterile water to final volume of 1 L.

Add DAPI 10mg/ml 1X soln.