The Cell Analysis Facility is located in the Department of Immunology
in Room H-581 HSC. The facility provides a broad range of instrumentation
and expertise for addressing experimental issues related to single cells.
The Facility makes available state-of-the-art instrumentation and techniques
while developing new ways of answering questions about the nature and
function of cells. Not only is instrumentation available to the University
community, but it is intended that the Facility contribute to all aspects
of the research process. This includes consultation in experimental design,
technical assistance in the operation of the instruments, distribution
of raw data files, archival data storage,
offline data analysis workstations, interpretation of results,
and the provision of facilities for the preparation and production of
presentation graphics.
The facility hosts a server that provides over 1TB of on-line access to link investigators and their laboratories to their collected data from anywhere using FTP. Workstations for data analysis are available in the computer lab free of charge.
For instruction, introduction to flow cytometry courses are offered periodically, and individual instruction can be provided as needed. Please call Michele Black at 543-7006 for details.
For details of availability, time, and charges, please see the section
on How to Use the Facility.
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IMPORTANT ANNOUNCEMENTS
1. Please note that the facility operates as a BSL-1 laboratory. Any material requiring BSL-2 containment is not permitted unless fixed. Any viable cells that could be classified as BSL-2 (this is all human cell lines and primary human samples ) must be cleared through EH&S by submitting a Research Project Hazard Assessment (RPHA) form prior to being sorted at facility.
When signing up for a sort please indicates the cells origin and you must specify the Biosafety level of your material. If it falls under BSL-2 you may not sort unless approved.
Approval will be given on a case by case basis through EH&S depending on the risk assessment as it applies specifically to cell sorting in the Cell Analysis Facility. Contact rbso@u.washington.edu to obtain the necessary forms and instructions to add the Cell Analysis Facility to your approved protocol.
2. The new Aria Cell sorter (Aria 2) is configured very similarly to the Aria 1 butthere are some minor differences that may require adjusting your settings in order for your samples to be properly compensated. Please speak with Michele or David if you are having issues or need assistance in setting up.
3. In order to maintain a clean and safe environment, please do not enter the facility with gloves from your lab. If you need gloves once inside the facility they are available by the sink in the analysis room and by each sorter in the sorting room. Gloves should be worn when disposing of sheath waste, gloves removed when finished and hands washed. Please DO NOT wear gloves or bring samples into the computer room. Samples can be placed on the counter next to Canto 2 or put in the small refrigerator under the bench by the FACScan while you are using the computers.
If you have any questions or any concerns please contact Michele Black at mblack2@u.washington.edu, or 685-3014.
