Instrumentation
All of the cytometers are capable of performing the
following:
- Multi- flurochrome analysis
- Cellular kinetics and physiological cellular responses
- DNA, RNA content, cell cycle, DNA synthesis
- Measurement of fluuorescent proteins
Analyzers
BD FACScan
3 Color Analyzer
Equipped with a 488 argon laser. Capable of 8 parameter data acquisition
including size, complexity, 3 fluorochromes as well as width and area
processing (used for DNA cell cycle analysis). An analog instrument
using Cell Quest software.
Optical Configuration Diagrams
BD FACSCanto (2 units)
6 Color Analyzer
Equipped with a 488nm argon laser and 633nm red diode laser. Capable
of simultaneous detection of 6 fluorochromes and 8 parameter data acquisition.
A digital instrument using FACSDiva software.
Optical Configuration Diagrams
BD LSR II
13 Color Analyzer
Equipped with a 488 nm argon laser, 633nm red diode laser, 405nm violet
laser, and 355 nm UV laser. Capable of simultaneous detection of 13
fluorochromes and 15 parameter data acquisition. A digital instrument
using FACSDiva software. The HTS plate reader accommodates 96-well plates for more automated acquisition of samples.
Optical Configuration Diagrams
Sorters
BD FACS Aria II (2 units)
13* Color High speed 4-way Sorter
The FACS Aria has been redesigned for better stability and ease of use. We have upgraded our existing system to take advantage of most of the improvements of the Aria II. The upgrade makes changing pressure and nozzle size significantly quicker and more reliable. The Aria II combines the sensitivity of a digital bench-top analyzer with the functionality of a high precision sorter. Improvements in fludics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with less stress on the sorted cells and enhanced overall sensitivity. Aria 1 uses fiber optic guided solid state 488nm, 635nm and 407nm diode lasers along with fiber guided emission collections (*Currently five colors plus scatter off the 488nm, two colors off the 635nm line and two off the 407nm line. As more dyes are developed the instrument has the ability to expand to accommodate more colors for each of the laser lines.) Aria2, our newest instrument, has the added improvement of direct laser alignment to the flow cell eliminating the fiber optics while conserving more power from the source. It also offers a 640nm 100mw for better sensitivity of red dyes, the 405nm has variable power to enhance such dyes as Pacific Blue and Am Cyan.
Automatic compensation improves the reproducibility of data by mathematically calculating spectral ov erlap. **See Aria Sample Prep for information on what controls are needed and a guide to optimize samples for sorting. Both sorters are equipped with an aerosol management system designed to decrease the risk of operating in a jet air sorter. (This DOES NOT imply that human infectious materials are permitted in the facility. Any unscreened human samples or pathogen containing samples need to be fixed (see Policies). This is a digital instrument using FACSDiva solfware.
Optical Configuration Diagram
Updated 6/3/09
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