Instrumentation

All of the cytometers are capable of performing the following:

• Multi- flurochrome analysis
  
  
• Cellular kinetics and physiological cellular responses
  
  
• DNA, RNA content, cell cycle, DNA synthesis
  
  
• Measurement of fluuorescent proteins

Analyzers

BD FACScan

3 Color Analyzer
Equipped with a 488 argon laser. Capable of 8 parameter data acquisition including size, complexity, 3 fluorochromes as well as width and area processing (used for DNA cell cycle analysis). An analog instrument using Cell Quest software.
Optical Configuration Diagrams

BD Calibur

BD FACSCanto

6 Color Analyzer
Equipped with a 488nm argon laser and 633nm red diode laser. Capable of simultaneous detection of 6 fluorochromes and 8 parameter data acquisition. A digital instrument using FACSDiva software.
Optical Configuration Diagrams

BD LSR

6 Color Analyzer
Equipped with a 488nm argon laser, red diode laser and HeCd UV laser. Capable of simultaneous detection of 6 fluorochromes and 8 parameter data acquisition. An analog instrument using Cell Quest software.
Optical Configuration Diagrams

BD LSR II

13 Color Analyzer
Equipped with a 488 nm argon laser, 633nm red diode laser, 405nm violet laser, and 355 nm UV laser. Capable of simultaneous detection of 13 fluorochromes and 15 parameter data acquisition. A digital instrument using FACSDiva software. The HTS plate reader accommodates 96-well plates for more automated acquisition of samples.
Optical Configuration Diagrams

Sorters

BD FACS Aria

13* Color High speed 4-way Sorter
This is our newest instrument. The FACS Aria combines the sensitivity of a digital bench-top analyzer with the functionality of a high precision sorter. Improvements in fluidics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with less stress on the sorted cells and enhanced overall sensitivity. The system uses fiber optic guided solid state 488nm, 635nm and 407nm diode lasers along with fiber guided emission collections (* Currently, five colors plus scatter off the 488nm, two colors off the 635nm line and 2 off the 407nm line. As more dyes are developed the instrument has the ability to expand to accommodate more colors for each of the laser lines.). Automatic compensation improves the reproducibility of data by mathematically calculating spectral overlap. See Aria Sample Prep for information on what controls are needed and a guide to optimize samples for sorting. The sorter is equipped with an aerosol management system designed to decrease the risk of operating in a jet air sorting system. (This DOES NOT imply that human infectious materials are permitted in the facility. Any unscreened human samples or pathogen containing samples need to be fixed (see Policies). A digital instrument using FACSDiva software.
Optical Configuration Diagram

BD FACS Vantage SE

6 Color High Speed Sorter
Currently equipped with Lyt200-S solid state 488nm laser, and a HeNe 633nm laser. The sorter is configured to collect in a variety of tubes and plates. The system is routinely run with a 70um tip, which accommodates most cell types as long as they are prepared properly in a single cell suspension (see Protocols). If cells larger than 25um are to be sorted, it is advisable to sign up for the Aria and request the 100um nozzle. The sorter is equipped with an aerosol management system designed to decrease the risk of operating in a jet air sorting system. (This DOES NOT imply that human infectious materials are permitted in the facility. Any unscreened human samples or pathogen containing samples need to be fixed (see Policies). An analog instrument using Cell Quest software.
Optical Configuration Diagram

Updated 5-18-07