Protocols

Buffers

NTS Buffer/DAPI Stain
RBC Lyses Solutions and Cell Lyses Procedure

Cell Preparation & Staining

Antibody Titration Protocol
Vantage Cell Prep for Sorting
Aria Cell Prep for Sorting
DNA Content Using Hoechst 33342 in Unfixed Cells
Cell Cycle Analysis Using Propidium Iodide (PI)

Software and Cytometer Instructions

Cytometer Setup FACScan & LSR
LSRII Startup
LSRII Shutdown
Data Retrieval from anywhere w/ FTP
Data Retrievan from local computers

FlowJo MAC

Updated 5-21-07