Daniel J. Campbell, Ph.D.
Affiliate Assistant Professor, Immunology

Benaroya Research Institute at Virginia Mason
1201 Ninth Avenue
Seattle, WA 98103
Tel: 206.341-0789
Fax: 206.233-7543
Email: campbell@benaroyaresearch.org

Dr. Campbell received his PhD in Molecular and Cell Biology at the University of California, Berkeley in 1998. Following a postdoctoral fellowship at Stanford, he joined the Benaroya Research Institute as Assistant Member in 2003.

Research Focus

The cells and tissues of the immune system are precisely organized to ensure the proper development, activation, function and regulation of diverse lymphocyte populations. Tissue and microenvironment selective lymphocyte homing is the basis for this organization, which in turn is mediated by lymphocyte expression of specific combinations of surface adhesion and chemoattractant receptors. Expression of these homing receptors therefore defines functionally specialized lymphocyte populations with unique tissue-tropisms.

We are interested in further exploring the relationship between lymphocyte function, homeostasis and localization. Using mouse models of autoimmunity, our goals are to track the differentiation and localization of various homing receptor-defined populations of CD4+ T cells, and to determine how each of these contributes to the function and regulation of immune responses in specific tissues. In addition, we seek to understand the signaling events and transcriptional networks that direct T cell expression of different homing receptor combinations, and therefore control ‘organ-specific' immunity and autoimmunity. Our work promises to yield new insights into lymphocyte differentiation and function, and holds great potential for the therapeutic manipulation of lymphocyte responses in the context of chronic infection, autoimmunity and transplantation.

Students studying in Dan Campbell's laboratory: Meghan Koch, Justin Killebrew

Selected Publications

Sather BD, Treuting P, Perdue N, Miazgowicz M, Fontenot JD, Rudensky AY, Campbell DJ. 2007. Altering the distribution of Foxp3+ regulatory T cells results in tissue-specific inflammatory disease. Journal of Experimental Medicine 204(6):1335-47.

Humblet-Baron S, Sather B, Anover S, Becker-Herman S, Kasprowicz DJ, Khim S, Nguyen T, Hudkins-Loya K, Alpers CE, Ziegler SF, Ochs H, Torgerson T, Campbell DJ, Rawlings DJ. 2007. Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis. Journal of Clinical Investigation 117(2):407-18.

Arnold CN, Campbell DJ, Lipp M, Butcher EC. 2007. The germinal center response is impaired in the absence of T cell-expressed CXCR5. European Journal of Immunology 37(1):100-9.

Rudensky AY, Campbell DJ. 2006. In vivo sites and cellular mechanisms of T reg cell-mediated suppression. Journal of Experimental Medicine 203(3):489-92.

Yoo, J., M. Omori, D. Gyarmati, B. Zhou, T. Aye, D.G. Branstatter, M.R. Comeau, D.J. Campbell, S. F. Ziegler. 2005. Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin (TSLP) transgene specifically in the skin. Journal of Experimental Medicine 202(4):541-9.

Zhou, B., M.R. Comeau, T. De Smedt, H.D. Liggitt, M.E. Dahl, D.B. Lewis, D. Gyarmati,T. Aye, D.J. Campbell, and S.F. Ziegler. 2005. Thymic stromal lymphopoietin as key initiator of allergic airway inflammation in mice. Nature Immunology 6(10):1047-53.

Arnold, C.N., E.C. Butcher, and D.J. Campbell. 2004. Antigen-specific lymphocyte sequestration in lymphoid organs: lack of essential roles for (alpha)L and (alpha)4 integrin-dependent adhesion or G(alpha)i protein coupled receptor signaling. Journal of Immunology. 173(2):866-73.

Staton, T.L., W.B. Johnston, E.C. Butcher, and D.J. Campbell. 2004. Murine CD8+ recent thymic emigrants are aE integrin+ and CCL25 responsive. Journal of Immunology. 172(12):7282-88.

Campbell D.J., G.F. Debes, W.B. Johnston, E. Wilson, and E.C. Butcher. 2003. Targeting T cell responses by selective chemokine receptor expression. Seminars in Immunology. 15(5):277-86.

Campbell D.J., and E.C. Butcher. 2002. Rapid acquisition of tissue-specific homing phenotypes by CD4+ T cells activated in cutaneous or mucosal lymphoid tissues. Journal of Experimental Medicine 195(1):135-41.

Campbell D.J., C.H. Kim, and E.C. Butcher. 2001. Separable effector T cell populations specialized for B cell help or tissue inflammation. Nature Immunology 2(9):876-81.

Kim C.H., L.S. Rott, I. Clark-Lewis, D.J. Campbell, L. Wu, and E.C. Butcher. 2001. Subspecialization of CXCR5+ T cells: B helper activity is focused in a germinal center-localized subset of CXCR5+ T cells. Journal of Experimental Medicine 193(12):1373-81.

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Updated 6/28/07