Wilson Lab : Christopher Wilson | Members | Research Interests | Reagent Requests| Protocols  

Wilson Lab: Reagent Requests - Constructs & Transgenic Mice

Reagents:

Reagents described in publications from our laboratory are available for distribution to investigators in academic laboratories for non-proprietary purposes. These include plasmids and similar reagents and transgenic mouse strains. Investigators wishing to receive a specific reagent should download and complete the Materials Transfer Agreement Form (PDF) relevant to their request. This can be mailed or faxed along with a specific letter of request to:

Christopher B. Wilson, M.D.
Department of Immunology, Box 357650
University of Washington
1959 NE Pacific St
Seattle, WA 98195
Phone 206-685-3956
FAX 206-616-4561
email cbwilson@u.washington.edu

Transgenic Mice:

Investigators wishing to receive lckCre or CD4Cre mice (as described in Lee PP, Fitzpatrick DR, Beard C, Jessup HK, Lehar S, Makar, KW, Perez-Melgosa, M, Sweetser, MT, Schlissel, MS, Nguyen S, Cherry SR, Tsai, JH, Tucker S,
Weaver, WM, Kelso A, Jaenisch R, Wilson CB. A critical role for Dnmt1 and DNA methylation in T cell development, function and survival. Immunity 15:763-774, 2001) are asked to contact Elizabeth Majane at NIAID, who will provide an MTA and arrange for mice to be sent from Taconic, where they have been deposited. If you are unable to get these mice from them, or have a colleague to whom we have sent the mice who could provide them to you, or you are requesting other transgenic mice developed in our laboratory, you may download the following Materials Transfer Agreement form (PDF), complete the form, and forward two copies to the above address/fax with a cover letter requesting permission to obtain the mice.

It has come to my attention that some investigators using the Lck-Cre mice have seen not only high-level deletion in double-negative thymocytes but partial to complete deletion in non-lymphoid tissues in some animals. This is not evident in most crosses to mice with loxP-flanked alleles, even in laboratories in which it has been seen in other crosses. In crosses and generations in which it has been observed, the frequencies have ranged from 0-~50% of the offspring. This has been confirmed in a recent complex cross in our laboratory. These occurrences are unpredictable and suggest variegated aberrant expression of the transgene in non-lymphoid contexts in some genetic backgrounds. To date, when this occurs it has been apparent in tail DNA samples. You should determine whether this occurs in your crosses and interpret the results accordingly. Aberrant deletion has not been observed with the CD4-Cre transgenic mice.