Wilson Lab: Reagent Requests - Constructs & Transgenic Mice

Reagents:

Dr. Wilson has left the University of Washington and is now at the Bill and Melinda Gates Foundation.

Reagents described in publications from the Wilson laboratory are available for distribution to investigators in academic laboratories for non-proprietary purposes. These include plasmids and similar reagents and transgenic mouse strains. Investigators wishing to receive a specific reagent should fill out and submit a request for Materials Transfer Agreement.

For questions regarding reagents, please contact Dr. Lynn Hajjar:

Lynn Hajjar, D.V.M., PhD
Department of Comparative Medicine, Box 357190
University of Washington
1959 NE Pacific St
Seattle, WA 98195
Phone 206-685-3733
FAX 206-685-3006
email hajjar@u.washington.edu

Transgenic Mice:

Investigators wishing to receive lckCre or CD4Cre mice (as described in Lee PP, Fitzpatrick DR, Beard C, Jessup HK, Lehar S, Makar, KW, Perez-Melgosa, M, Sweetser, MT, Schlissel, MS, Nguyen S, Cherry SR, Tsai, JH, Tucker S,
Weaver, WM, Kelso A, Jaenisch R, Wilson CB. A critical role for Dnmt1 and DNA methylation in T cell development, function and survival. Immunity 15:763-774, 2001) are asked to contact Elizabeth Majane at NIAID, who will provide an MTA and arrange for mice to be sent from Taconic, where they have been deposited. If you are unable to get these mice from them, or have a colleague to whom we have sent the mice who could provide them to you, or you are requesting other transgenic mice developed in our laboratory, you may fill out and submit the online request for Materials Transfer Agreement.

It has come to my attention that some investigators using the Lck-Cre mice have seen not only high-level deletion in double-negative thymocytes but partial to complete deletion in non-lymphoid tissues in some animals. This is not evident in most crosses to mice with loxP-flanked alleles, even in laboratories in which it has been seen in other crosses. In crosses and generations in which it has been observed, the frequencies have ranged from 0-~50% of the offspring. This has been confirmed in a recent complex cross in our laboratory. These occurrences are unpredictable and suggest variegated aberrant expression of the transgene in non-lymphoid contexts in some genetic backgrounds. To date, when this occurs it has been apparent in tail DNA samples. You should determine whether this occurs in your crosses and interpret the results accordingly. Aberrant deletion has not been observed with the CD4-Cre transgenic mice.