This year, ISCRM launched its first image contest that gave ISCRM researchers the opportunity to display their work using images that were taken using the Garvey Imaging core microscopes.The winning images are being professionally printed and displayed in ISCRM hallways. A selection panel at ISCRM comprised of scientists and staff evaluated entries and selected the top five images.

 

First Place

   

Top Image: Danny Omar El-Nachef (Robb MacLellan lab / now in the Davis and Sniadecki labs)    

A 3D reconstruction of adult mouse liver showing 3 slices across the volume. Hepatocytes are outlined in red, blood vessels are outlined in green, and nuclei are shown in blue. The central vein (large vessel) and the bile duct (medium vessel with red and green staining) run down the volume as parallel structures. Multinucleated hepatocytes can be seen.

Bottom Image: Christian Mandrychy (Zheng​ Lab)

This image captures one loop of an engineered spiral blood vessel coated with human umbilical vein endothelial cells. The blood vessel is ~3 mm in diameter, and this projected image captures almost 1 mm of depth as the vessel coils into the frame. The cell borders are in magenta (VECAD), nuclei in blue, and von Willebrand Factor (a factor important in hemostasis) in green. Engineered blood vessels like this allow us to better understand how the cells that line our blood vessels recognize and react to changes in their environment.

Third Place

Xiulan Yang (Chuck Murry Lab)

Cardiomyocytes derived from human embryonic stem cells grafted into an infarcted primate heart. Staining for human cardiac troponin I labels the cells of the graft (green). The host myocardium is shown in red. Collagen, a major component of the scar tissue after heart attack, is shown in blue.

Fourth Place

Allison Knupp (Jessica Young Lab)

Neurons grown from the brain tissue of a patient with Alzheimer's disease. Fibroblasts from the meninges were collected at autopsy and transdifferentiated directly into neurons. Growing neurons in this way retains aspects of their cellular age, helping us study diseases like Alzheimers that only manifest after long-term cumulative changes. 

Fifth Place

Jonathan Mene (Kelly Stevens Lab)

Spherical cell aggregates made from cocultures of human primary hepatocytes and dermal fibroblasts. The cells were labled for the hepatocyte marker CK18 (dark blue); the cholangiocyte marker CK19 (white) and nuclei (red). These cocultures help hepatocytes retain their functional properties in culture.

Honorable Mention

Jesse Macadangdang (Deok-Ho Kim Lab)

Cardiac cells plated on nanoscale grooves and ridges. The patterned growth substrate mimics the structure of the extracellular matrix and promotes cellular alignment and structural development. Actinin staining is shown in red, actin in green, and nuclei in blue.

Honorable Mention

Celina Gunnarsson (Ying Zheng Lab)

Engineered “capillaries” spanning large-diameter vessels. Capillary vessels were carved using a high-powered laser. Human endothelial cells line all vessels. Cell nuclei are shown in blue, the structural protein F-actin is shown in pink, and the blood-clotting protein von Willebrand Factor is shown in green. These capillary networks can help us study processes such as how red blood cells squeeze through capillaries and how blood diseases impair microcirculation.