Institute for Stem Cell & Regenerative Medicine

at the University of Washington

Lynn and Mike Garvey Cell Imaging Lab

Microscopes in the Garvey Cell Imaging Lab

Nikon A1R Confocal

The Nikon A1R confocal is suitable for generating optical sections of specimens labeled with up to four fluorescent probes -- blue, green, red, and far-red.  The confocal is mounted on a Nikon TiE inverted microscope with objective lenses available from 10X – 60X including oil and water immersion.  Suitable fixed specimens would be cultured cells and tissue sections up to 50 micrometers thick.  Available techniques include generation of serial optical sections for 3D reconstruction, automated creation of mosaic images of large specimens at high resolution, and live-cell studies using a stage-top incubator and focus maintaining device to prevent focal plane drift during long time-course experiments.
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Olympus FV1000 MPE BX61 Multi-photon Microscope

The Olympus multi-photon microscope is available for thick tissue (50 – 500 micrometers) and photo-sensitive live -cell imaging applications.  The multi-photon microscope uses infrared 2-photon excitation to generate optical sections for 3D reconstruction with increased penetration in thick specimens and reduced phototoxicity in live cells. The system is mounted on an Olympus BX 61 upright fixed stage microscope equipped with objective lenses suitable for imaging specimens immersed in aqueous buffer.  A Spectra-Physics Mai Tai high power laser capable of excitation at 690 - 1020 nm and suitable filtersets make this system optimized for imaging fluorescent proteins.
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Nikon TiE Inverted Widefield Fluorescence Microscopes

The software controlling these 2 systems allows both straightforward individual image collection as well as complex, automated experiments with multiple stage positions, wavelength changing, and multiple time points. Complete interchangeability of components allows these systems to be configured to suit a wide variety of specimens and experimental techniques.

The High-Resolution System is optimized for general, high-resolution fluorescent microscopy of fixed cells and tissues. Filtersets are available for Blue, Green, Red, and Far Red fluorescent probes.  Objective lenses span 4X – 60X and the system has a very high resolution CCD camera.  A motorized filter changer and motorized XY stage makes this system ideal for collecting image sets suitable for deconvolution as well as automated creation of mosaic images of large specimens at high resolution.
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The Live-Cell System is very similar to the high-resolution system, but has been optimized for live-cell studies.  This system utilizes a high sensitivity EMCCD camera capable of rapid frame rates, a piezo focus drive for rapid and precise collection of z-stacks, and a microscope enclosure for environmental control (temp and CO2/mixed gasses) of the specimen. A focus maintaining device prevents focal plane drift during long time-course experiments.  Filtersets have been chosen for imaging fluorescent proteins (CFP, GFP, YFP, and RFP).
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