Denitrifier_Lab1_Response.txt

Bottle = 4/5 medium 1/5 headspace.  Something to do with oxygen  
sensitivity during accumulation of nitrogen oxides.

There should be absolutely NO oxygen leaking into the bottle during  
growth on the shaker.  Otherwise the cells get killed off from the  
formation of free radicals (presumably, reactions with NO).

Our success rate is ~90%.  Clumped cells are usually indicative of  
toxicity, which we think is indirectly mediated by oxygen-NOx chemistry.

Make sue you have no contaminating strain in the stock cultures.