Denitrifier_Lab6_Response.txt

Our original starter culture of P. aureofaciens was taken (ANONYMOUS LAB) in ~2005.  We also purchased our own 
stock which is stored in the (ANONYMOUS DEPARTMENT) (in glycerine? In 2008 
or 2009, we tried to regrow from the freezer stock,  but they didn't 
work (the technician used a different broth mix). We didn't try again,  
but instead continue to innoculate from vials which have their origin 
from the (SAME ANONYMOUS LAB).

In answer to your questions:

1) Never
2) Never
3) n/a
4) n/a
5) n/a
6) we innoculate from starter cultures (from 100 ml serum vials to fresh 
vials of broth).
7) we follow Signman exactly
8) 125 ml Serum vials (VWR #16171-385)
9) 6
10) room temperature on the lab bench, no special precautions regarding 
light
11) 122 oC for 20 minutes
12) manual shaking
13) sometimes but usually they are consistent size and amount
14) so far 100% success.  We've not had a bad batch yet