DeltaVision Instructions

	Keck Microscopy Facility
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DELTAVISION-RT LIVE-CELL FILTER SETS

CFP -- Shown with excitation (dashed) and emission (solid) spectra for CFP.

DV-RT CFP

GFP -- Shown with excitation (dashed) and emission (solid) spectra for eGFP.

DV-RT GFP

YFP -- Shown with excitation (dashed) and emission (solid) spectra for eYFP.

DV-RT YFP

RFP_DBL -- Shown with excitation (dashed) and emission (solid) spectra for Alexa568 (similar to mCherry).

DV-RT RFP_DBL

RFP_TPL -- Shown with excitation (dashed) and emission (solid) spectra for Alexa568 (similar to mCherry).

DV-RT RFP_TPL

Here's the overlaid spectra for GFP/Alexa568 (similar to mCherry) using GFP/RFP_DBL filters.

DV-RT GFP-RFP_DBL

Here's the overlaid spectra for CFP/YFP/Alexa568 (similar to mCherry) using CFP/YFP/RFP_TPL filters.

DV-RT CFP-YFP-RFP_TPL

Spectra generated using Molecular Probe's Spectra Viewer

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

Keck Microscopy Facility