Keck Microscopy Facility

focus 

Keck Microscopy Facility Homepage

Objectives on the Nikon Inverted Microscope

 

The following objectives are on the Nikon Inverted microscope:


10X Plan N.A. 0.25 Phase
20X Plan N.A. 0.40 Phase, Long-working distance
20X PlanApo N.A. 0.75
40X Fluor N.A. 0.85
40X Fluor N.A. 1.30 oil immersion lens

60X Plan Apo N.A. 1.40 oil immersion lens

Notes:

The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.

 

 

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2007 Keck Microscopy Facility, University of Washington