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Objectives on the Nikon Inverted Microscope


The following objectives are on the Nikon Inverted microscope:

10X Plan N.A. 0.25 Phase
20X Plan N.A. 0.40 Phase, Long-working distance
20X PlanApo N.A. 0.75
40X Fluor N.A. 0.85
40X Fluor N.A. 1.30 oil immersion lens

60X Plan Apo N.A. 1.40 oil immersion lens


The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.



Contact Greg Martin at for additional information. Suggestions and comments are always welcome!

© 2007 Keck Microscopy Facility, University of Washington