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Objectives on the Nikon Inverted Microscope


The following objectives are on the Nikon Inverted microscope:

10X Plan N.A. 0.25 Phase
20X Plan N.A. 0.40 Phase, Long-working distance
20X PlanApo N.A. 0.75
40X Fluor N.A. 0.85
40X Fluor N.A. 1.30 oil immersion lens

60X Plan Apo N.A. 1.40 oil immersion lens


The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.



Contact Nathaniel Peters at ncpeters@uw.edu for additional information. Suggestions and comments are always welcome!

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