W. M. Keck Microscopy Center
Objectives on the Nikon Inverted Microscope
The following objectives are on the Nikon Inverted microscope:
10X Plan N.A. 0.25 Phase
20X Plan N.A. 0.40 Phase, Long-working distance
20X PlanApo N.A. 0.75
40X Fluor N.A. 0.85
40X Fluor N.A. 1.30 oil immersion lens
60X Plan Apo N.A. 1.40 oil immersion lens
The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.
Contact Nathaniel Peters at email@example.com for additional information. Suggestions and comments are always welcome!
© 2016 W. M. Keck Microscopy Center, University of Washington