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Objectives on the Nikon Upright Microscope


The following objectives are on the Nikon Upright microscope:

2X Plan N.A. 0.06
4X Plan Fluor N.A. 0.13
10X Plan Fluor N.A. 0.30
20X Plan Apo N.A. 0.75
40X S Fluor N.A. 0.90

60X Plan Apo N.A. 1.40 oil immersion lens

A 1X Plan N.A. 0.04 is also available -- ask Greg to put it on if you want to use it.


The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.



Contact Greg Martin at for additional information. Suggestions and comments are always welcome!

© 2007 Keck Microscopy Facility, University of Washington