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Widefield Microscopes  
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Widefield Microscopes Available at the Keck Center

Widefield microscopes are what most people think of as "standard" microscopes.

These are the most straightforward microscopes to use and should be the starting point for all of your microscopy projects.

There are three widefield microscopes available at the Keck Center, all in room K507B:

 

Nikon Upright (Nikon Eclipse E600)

Most basic microscope at the Keck Center.

Suitable for all specimens mounted on slides and coverslipped.

Excellent series of objectives from 1X - 60X

Filtersets for fluorescence imaging with standard blue, green, and red fluorophores

100 W mercury arc-lamp excitation source

DIC (Nomarski) and standard brightfield available

QImaging Retigia EX cooled CCD camera
--8 or 12-bit monochrome imaging
-- LCD filter available for color imaging

Click Here for Instructions

 

 

 

Nikon Inverted (Nikon Diaphot 200)

 

Non-infinity corrected objectives 5X - 100X suitable for all specimens mounted on slides and coverslipped.

Long working-distance objectives available for imaging specimens in culture dishes

Sutter filter wheel for automated and rapid switching of excitation wavelengths

Filters for fluorescence imaging with standard blue, green, and red fluorophores; FURA-2; CFP/YFP FRET

Phase contrast and Hoffman Modulation Contrast available

Princeton Instruments MicroMax interline transfer cooledCCD camera for rapid 12-bit imaging.

Filter wheels and camera operated by MetaMorph for automation of image aquisition

Click Here for Instructions

 

 

 

 

 

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2005 Keck Imaging Center, University of Washington