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Objectives on the Zeiss META Confocal Microscope


The following objectives are on the Zeiss confocal microscope:

10X PLAN APO N.A. 0.45
20X PLAN APO N.A. 0.75
40X PLAN NEOFLUAR N.A. 1.30 Oil Immersion
N.A. 1.40 Oil Immersion
100X PLAN-FLUAR N.A. 1.45 Oil Immersion

63X C-APO N.A. 1.20 Water Immersion

A 5X FLUAR N.A. 0.25 is also available -- ask Nathaniel to put it on if you want to use it.



The 63X water immersion objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.



Contact Nathaniel Peters at for additional information. Suggestions and comments are always welcome!

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