W. M. Keck Microscopy Center
Objectives on the Zeiss META Confocal Microscope
The following objectives are on the Zeiss confocal microscope:
10X PLAN APO N.A. 0.45
20X PLAN APO N.A. 0.75
40X PLAN NEOFLUAR N.A. 1.30 Oil Immersion
63X PLAN APO N.A. 1.40 Oil Immersion
100X PLAN-FLUAR N.A. 1.45 Oil Immersion
63X C-APO N.A. 1.20 Water Immersion
A 5X FLUAR N.A. 0.25 is also available -- ask Nathaniel to put it on if you want to use it.
The 63X water immersion objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.
Contact Nathaniel Peters at firstname.lastname@example.org for additional information. Suggestions and comments are always welcome!
© 2016 W. M. Keck Microscopy Center, University of Washington