Keck Microscopy Facility
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Keck Microscopy Facility |
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The UV/VIS collimator needs to bet set to provide correct Z-axis registration between the 405 nm excitation and the visible laser excitation wavelengths (488, 543, 633 nm).
From the main Zeiss toolbar
Select Maintain
Select Pinhole
From the Pinhole and Collimator window
Do not adjust pinhole settings.
Under Collimator
Select UV/VIS
In the Collimator window
Input the following value, according to the objective lens being used, in the Position [mm] window:
0.05 for the 10X
-0.25 for the 20X
-1.25 for the 40X oil
-2.40 for the 63X oil
-1.30 for the 63X water
Do not use the 405 nm laser in combination with the visible lasers with the 100X oil objective.
Close the Pinhole and Collimator window. Your selection will be maintained.
Return to "Acquire".
Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!
© 2007 Keck Microscopy Facility, University of Washington