Keck Imaging Center

Zeiss Confocal Instructions: Startup 
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If the system is off, follow these instructions. Turn on only the lasers you need.

If the system is already running, check that the lasers you need are turned on.

Zeiss Confocal

Log onto the computer: Use you Keck Center Username and password. If the computer is not on, check that the SYSTEM/PC switch ((1) on the image below) is on. If needed, turn on the computer with this switch. If the computer is still off, turn it on by pressing the power button on the computer.

Power up the system:

Zeiss switchbox

The switch (1) for the computer (SYSTEM/PC) should always be on. If this switch is on and the computer is still off, turn it on by pressing the power button on the computer.

Turn on the COMPONENTS switch (2) This will power up the microscope, scan electronics and laser control box.

 

 

 

 

 

 

 

Turning on the mercury lamp:

Zeiss Hg Lamp Power supply

Turn on the mercury lamp at the power supply (3).

The FluoArc Control Pad (4) will look like the image below when the lamp is on.

Zeiss Hg Lamp Control Pad

 

Press the F2 button to change the intensity to 50%. This should be a suitable intensity for most specimens. Decrease or increase the intensity with the F3 or F4 buttons if needed.

The lamp should remain on for at least one hour. If turned off, it should cool for at least 20 minutes before turning on again.

DON'T ADJUST THE KNOBS ON THE MICROSCOPE LAMPHOUSE.
Ask Greg for show you how to align the lamp if you think it's misaligned.

Anti-vibration Table:

Zeiss N2 TankOpen the valve on the top of the N2 cylinder (5). The pressure should read 60 PSI on the gauge (6). If there is no pressure, change the regulator (7) to the other cylinder (8) and let Greg know.

The top of the anti-vibration table should rise slightly.

 

 

 

 

 

 

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2005 Keck Imaging Center, University of Washington