Keck Microscopy Facility
Zeiss Confocal Instructions: Startup
Sign in on the log book
If the system is off, follow these instructions. Turn on only the lasers you need.
If the system is already running, check that the lasers you need are turned on.
Log onto the computer: Use you Keck Center Username and password. If the computer is not on, check that the SYSTEM/PC switch ((1) on the image below) is on. If needed, turn on the computer with this switch. If the computer is still off, turn it on by pressing the power button on the computer.
Power up the system:
The switch (1) for the computer (SYSTEM/PC) should always be on. If this switch is on and the computer is still off, turn it on by pressing the power button on the computer.
Turn on the COMPONENTS switch (2) This will power up the microscope, scan electronics and laser control box.
Turning on the mercury lamp:
Turn on the mercury lamp at the power supply (3).
The FluoArc Control Pad (4) will look like the image below when the lamp is on.
Press the F2 button to change the intensity to 50%. This should be a suitable intensity for most specimens. Decrease or increase the intensity with the F3 or F4 buttons if needed.
The lamp should remain on for at least one hour. If turned off, it should cool for at least 20 minutes before turning on again.
ADJUST THE KNOBS ON THE MICROSCOPE LAMPHOUSE.
Ask Nathaniel for show you how to align the lamp if you think it's misaligned.
Open the valve on the top of the N2 cylinder (5). The pressure should read 60 PSI on the gauge (6). If there is no pressure, change the regulator (7) to the other cylinder (8) and let Nathaniel know.
The top of the anti-vibration table should rise slightly.
Contact Nathaniel Peters at firstname.lastname@example.org for additional information. Suggestions and comments are always welcome!
© 2015 Keck Microscopy Facility, University of Washington