CCD Imaging

Instructions for shutting down the system

1. Turn on the xenon light first - turn on the POWER on the LPS-220 PTI Arc Lamp Supply (right side of microscope).

Other settings:Igniter is on auto - do not use the START button
When the selector is on AMPS the display should be 5.00. Adjust the current if too high or low.
Total lamp time should not be more than 440 hours.

2. Turn on the power stip (left side of microscope).

3. Flip the REMOTE START lever forward (on the small black Metaltek box). You should hear the filter wheel initialize).

4. Select button #8 on the Metaltek box.

5. Flip the SHUTTER on (you should see light at the microscope) or off to block the light.

6. Select the filter block under the objective turret - slide it in for green excitation, red emission or out for blue excitation, green emission.

Other microscope settings:There are two neutral density sliders (10% and 25% transmission) than can be used on the back of the microscope if the intensity of the excitation light is too strong (i.e.bleaches your sample)
Bi/Photo slider on the right side of the microscope must be in (Cine/Photo doesn't matter).

Instructions for shutting down the system:

LEAVE THE LAMP ON FOR AT LEAST ONE HOUR

1. turn off the power strip on the left side of the microscope.

2. Turn off the POWER on the LPS-220 lamp Supply.

3. Lower the objective focus.

Equipment:

Nikon Diaphot TMD microscope

inverted
objectives available

A MetalTek shutter and filter wheel is directly attached to the microscope.

Filters:

Fura: 340/10, 380/13 nm filters are in the filter wheel. Dichroic and emission (535/50) filters are in the filter block that must be inserted below the objective turret.

FITC: all filters are in the 2-block filter carrier that must be inserted below the objective turret. Excitation 480/30, dichroic 505DCLP, and emission 535/40 filters are in the block.

TRITC: all filters are in the 2-block filter carrier that must be inserted below the objective turret. Excitation 540/25, dichroic 565DCLP, and emission 605/55 filters are in the block.

To view double labels the FITC and TRITC blocks must be switched. There is displacement when doing this so this system should not be used for double labels. A set up for double labeling is on the Nikon/PI system in K507B.

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