Instructions for starting the system
Instructions for using the software
Instructions for shutting down the system
Equipment:
Olympus microscope:
Princeton Instruments PentaMax 512EFT camera:
TILL monochromater
Filters:
Excitation:
TILL monochromater provides wavelengths from 260-680 nm. with a bandwidth of 15nm.Dichroic and Emission filters:
Note: All excitation and emission filters from Chroma are marked with an arrow. The arrow should point to the inside of the cube. Dichroic filters are oriented with the coated side toward the excitation filter.
| Fluorophore | Dichroic (Beamsplitter) | Emission | Notes |
| FURA | 400DCLP | - | - |
| MEQ | 400DCLP | 420LP | - |
| Lucifer Yellow | 460DCLP | 500LP | - |
| Calcium Green | 515DCLP | 520LP | 400LP * |
| Calcium Crimson | 595DCLP | 600LP | - |
*A 400LP filter is installed in the excitor filter position. This prevents illuminating the sample when shuttering to a wavelength <400nm.
IR bandpass filters: 775/150 or 770/40
Instructions for starting the system
1. The xenon lamp must be ignited before anything else is turned on.
Turn on the red lamp switch on the Polychrome II.
Wait 30 seconds (you will hear the lamp ignite).
Turn on the green DCPower switch.
All of the voltage lights should come on.
The lamp should be changed at approx. 500 hours.
2. Turn on the power strip behind the video monitor. This will start the cameras, shutter and video monitor.
3. Turn on the on the power strip by the computer monitor to start the computer and monitor - this switch is also for the transmitted light.
4. A password is necessary to use the computer.
5. Select Windows95 from the System Commander menu.
6. A password is not necessary for Windows Networking.
7. Start the "Use this MetaFluor". Type in your name to set up a configuration file.
Instructions for using the software
Instructions for shutting down the system
Polychrome II monochromater: "There is certainly some stray light with the Polychrome II monochrometer. This is due mostly to light scattered off the grating, and the background level has little to do with which end the monochrometer is parked at. Tests have shown that about 2000 seconds of exposure at the resting wavelength of 250nm produces a FURA-emission equivalent to 1 second of exposure at 380nm."
Solution: