Keck Imaging Center

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Please read before training:
Why use a confocal microscope?

Which confocal microscope should you use?

Confocal or 2-photon microscopy?

Sample Preparation

Nikon MicroscopyU
Interactive java tutorials on widefield vs confocal microscopy, focus, gain, speed and aperture size


You should have an idea of how fluorescence works (http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html), what fluorophores you will be using, and the full excitation and emission spectra of the fluorophores (http://www.probes.com/, http://www.clontech.com/index.shtml, http://www.jacksonimmuno.com/).



 

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2006 Keck Imaging Center, University of Washington