W. M. Keck Microscopy Center

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Confocal Instructions

 

Confocal Information

Please read before training:

Why use a confocal microscope?

Which confocal microscope should you use?

Sample Preparation

Nikon MicroscopyU
Interactive java tutorials on widefield vs confocal microscopy, focus, gain, speed and aperture size


You should have an idea of how fluorescence works (http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html), what fluorophores you will be using, and the full excitation and emission spectra of the fluorophores (http://www.probes.com/, http://www.clontech.com/index.shtml, http://www.jacksonimmuno.com/).

 

Contact Nathaniel Peters at ncpeters@uw.edu for additional information. Suggestions and comments are always welcome!

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