W. M. Keck Microscopy Center
Please read before training:
Why use a confocal microscope?
Which confocal microscope should you use?
Interactive java tutorials on widefield vs confocal microscopy, focus, gain, speed and aperture size
You should have an idea of how fluorescence works (http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html), what fluorophores you will be using, and the full excitation and emission spectra of the fluorophores (http://www.probes.com/, http://www.clontech.com/index.shtml, http://www.jacksonimmuno.com/).
Contact Nathaniel Peters at firstname.lastname@example.org for additional information. Suggestions and comments are always welcome!
© 2016 W. M. Keck Microscopy Center, University of Washington