Keck Microscopy Facility
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Keck Microscopy Facility |
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Confocal Instructions
Confocal Information
Please read before training:
Why use a confocal microscope?
Which confocal microscope should you use?
Sample Preparation
Nikon MicroscopyUInteractive java tutorials on widefield vs confocal microscopy, focus, gain, speed and aperture size
You should have an idea of how fluorescence works (http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html), what fluorophores you will be using, and the full excitation and emission spectra of the fluorophores (http://www.probes.com/, http://www.clontech.com/index.shtml, http://www.jacksonimmuno.com/).
Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!
© 2007 Keck Microscopy Facility, University of Washington
