Keck Microscopy Facility

Objectives on the DeltaVision Microscope

The following objectives are on the DeltaVision deconvolution microscope:

20X UPlanApo N.A. 0.70
40X UApo N.A. 0.90
40X UPlanApo N.A. 1.35 Oil Immersion
60X PlanApo N N.A. 1.42 Oil Immersion
100X UPlanApo N.A. 1.35 Oil Immersion

A 10X UPlanApo N.A. 0.40 is also available -- ask Greg to put it on if you want to use it.

A 60X UPlanApo N.A. 1.20 water immersion lens is also available -- ask Greg to put it on if you want to use it.

Notes:

The 40X and 100X oil objectives have an iris adjusted with a collar on the barrel of the objective. The collar should be turned full clockwise.

The 40X dry objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.

The 60X water immersion objective has a cover slip correction collar. Cover slips vary in thickness with a # 1 1/2 coverslip being nominally .17 mm thick. Adjust the collar for the best image: while looking at the specimen by eye, adjust the collar in small increments, refocusing continuously with the microscope's fine focus. Go for the highest contrast, "sharpest" image -- a small punctate structure should become blurry in an identical fashion as you defocus both above and below the structure.

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2007 Keck Microscopy Facility, University of Washington