W. M. Keck Microscopy Center
Zeiss LSM 710 Scanning Confocal. This microscope collects optical sections of fluorescently-labeled specimens and has the following features:
- Manual stage with slide, dish, and plate inserts
- 10X and 20X dry, 40X, 63X, and 100X oil immersion, and 40X and 63X water immersion objectives, all with DIC prisms
- 7 fixed wavelength laser lines: 405, 458, 488, 514, 561, 594, and 633nm
- 3 confocal detectors: 2 standard PMTs and 1 spectral PMT
- Tunable emission windows for all detectors
- Trans-PMT detector for DIC imaging
- Zeiss Zen 2008 software
Leica SP8X Scanning Confocal. This microscope collects optical sections of fluorescently-labeled specimens and has the following features:
- Motorized XY stage capable of tiling and multi-point imaging
- Motorized Z stage for fast, precise movement in Z
- 10X and 20X* dry, 40X* and 63X* oil immersion, and 25X and 63X* water immersion objectives with DIC prisms*
- Adaptive focus control to maintain focus during live imaging and/or tilescanning
- Tunable White Light Laser system (470-670nm)
- 6 fixed wavelength laser lines: 405, 458, 476, 488, 496, and 514 laser lines
- 5 confocal detectors: 3 standard PMTs and 2 high-sensitivity HyD detectors with lifetime gating
- Trans-PMT detector for DIC imaging
- Resonant scanner
- Leica LASX software
Nikon Upright. This is a Nikon Eclipse E600 microscope with a QImaging Retigia EX CCD Camera for capturing B&W images of fluorescent specimens labeled with blue, green, or red fluorophores. A special liquid-crystal color filter is available for color imaging of histology slides. Click on the following links for available filters and objectives.
Leica Inverted. This is a Leica DMI6000 microscope with a Leica DFC365 FX CCD camera and motorized filters, stage, and focus. It has blue, green, red, and far red filter sets, and a wide range of objectives including long working distance Phase Contrast objectives for imaging through plastic culturing dishes/plates. It is is suitable for high-resolution deconvolution microscopy, image tiling for large areas, and can be outfitted with environmental control for live imaging applications.
Live Cell Chambers:
Warner PDMI-2 Open Perfusion Micro-Incubator. This is an open system that takes 35 mm coverglass-bottomed dishes. It can be used under static or perfusion conditions on the Leica SP8X Confocal, the Zeiss 710 Confocal, or the Leica Inverted Widefield system.
Pecon Stage Incubator. This temperature-controlled stage insert can control temperature in 35mm coverglass-bottomed dishes and/or coverglass-bottomed culture slides and has an optional gas-fed lid that can be used to maintain 5% CO2 conditions for longer culturing. It can be used on the Leica SP8X Confocal, the Zeiss 710 Confocal, or the Leica Inverted Widefield system.
Image Processing Computers:
There are two PC computers at the Keck Center available for image processing and analysis. Which one you choose will depend on what you are trying to accomplish and what software you are comfortable using. Every Keck Center user has access to these computers.
Analysis FIJI/ImageJ and LASX. This computer is a basic PC capable of processing 2D and many 3D datasets from all Keck Center microscopes. FIJI/ImageJ is free and highly capable image analysis software and LASX is the software that operates the Leica SP8X confocal microscope. This computer is free to use for images acquired at the Keck Center. Reservations are not required, but are recommended.
Analysis FIJI/IMARIS. This computer is a high-powered PC with 64GB of RAM and an 8GB graphics card. IMARIS is an advanced 3D/4D visualization and analysis program capable of handling large and complex datasets and is compatible with datasets from all Keck Center microscopes. This computer has an hourly rate associated with it and reservations are required.
Contact Nathaniel Peters at firstname.lastname@example.org for additional information. Suggestions and comments are always welcome!
© 2016 W. M. Keck Microscopy Center, University of Washington