Keck Microscopy Facility

focus 

Keck Microscopy Facility Homepage

Equipment

Confocal Microscopes:

Zeiss LSM 510 META. This microscope collects optical sections of fluorescently-labeled specimens. It has spectral detection to maximize its utility for multi-labeled specimens and is on an inverted microscope. Click on the following links for information about the available lasers, objectives, and emission filters.

Leica SL . This microscope collects optical sections of fluorescently-labeled specimens. It has spectral detection to maximize its utility for multi-labeled specimens and is on an upright microscope. Click on the following links for information about the available lasers and objectives.

Deconvolution (Image Restoration) Microscopes:

DeltaVision RT System. This deconvolution microscope is optimized for live-cell imaging.   It has a very sensitive and fast EM-CCD camera (Cascade II from Photometrics) and consists of an inverted microscope within a Plexiglas enclosure to keep the entire system (microscope, stage and specimen) at 37C and in a humidified CO2 environment if necessary.  Camera, filters, and stage are all controlled by an integrated software package with many features optimized for imaging living cells at rapid frame rates and/or over long time periods. Click on the following links for available standard or live-cell filters and objectives.

Widefield Microscopes:

Nikon Upright. This is a Nikon Eclipse E600 microscope with a QImaging Retigia EX CCD Camera for capturing B&W images of fluorescent specimens labeled with blue, green, or red fluorophores. A special liquid-crystal color filter is available for color imaging of histology slides. Click on the following links for available filters and objectives.

Leica Inverted. This is a Leica DMIRB microscope with a CoolSnap CCD camera and automated filters/stage, and focus. It is is suitable for high-resolution deconvolution microscopy and image tiling for large areas. Click on the following links for available filters and objectives.

Live Cell Chambers:

Warner PDMI-2 Open Perfusion Micro-Incubator. This is an open system that takes 35 mm coverglass-bottomed dishes. It can be used under static or perfusion conditions. It can be mounted on the Zeiss META confocal, the DeltaVision RT microscope, or the Leica Inverted.

Image Processing Computers:

There are two computers available for image analysis. Which one you choose will depend on what you are trying to accomplish and what software you are comfortable using. Every Keck Center user has access to these computers at no charge for analyzing images collected with the Keck Center's microscopes.

1) A PC running MetaMorph, a very powerful image analysis program. It is often the best choice as it will extract many kinds of data from your images for export to Excel. MetaMorph can be automated for repetitive tasks and works well with image stacks. This computer also has Photoshop and ImageJ.

2) A fast and powerful 64-bit windows PC running 3D visualization and analysis tools from Imaris that can be used on images from all of the Keck Facility microscopes.

 

 

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

© 2007 Keck Microscopy Facility, University of Washington