Equipment List

	Keck Microscopy Facility
Keck Microscopy Facility Homepage

Equipment

Confocal Microscopes:

Zeiss LSM 510 META. This microscope collects optical sections of fluorescently-labeled specimens. It has spectral detection to maximize its utility for multi-labeled specimens and is on an inverted microscope. Click on the following links for information about the available lasers, objectives, and emission filters.

Leica SP1. This microscope collects optical sections of fluorescently-labeled specimens. It has spectral detection to maximize its utility for multi-labeled specimens and is on an inverted microscope. Click on the following links for information about the available lasers and objectives.

Leica SL . This microscope collects optical sections of fluorescently-labeled specimens. It has spectral detection to maximize its utility for multi-labeled specimens and is on an upright microscope. Click on the following links for information about the available lasers and objectives.

Deconvolution (Image Restoration) Microscopes:

Deltavision System. This is an integrated system for the collection high-resolution z-series that are subsequently deconvolved to produce optical sections that are free from out-of-focus blur, similar in appearance to confocal optical sections. It is based on an Olympus inverted IX70 microscope. Click on the following links for available filter sets and objectives.
DeltaVision RT System. This deconvolution microscope is optimized for live-cell imaging. It has a very sensitive and fast EM-CCD camera (Cascade II from Photometrics) and consists of an inverted microscope within a Plexiglas enclosure to keep the entire system (microscope, stage and specimen) at 37C and in a humidified CO2 environment if necessary. Camera, filters, and stage are all controlled by an integrated software package with many features optimized for imaging living cells at rapid frame rates and/or over long time periods. Click on the following links for available standard or live-cell filters and objectives.

Widefield Microscopes:

Nikon Upright. This is a Nikon Eclipse E600 microscope with a QImaging Retigia EX CCD Camera for capturing B&W images of fluorescent specimens labeled with blue, green, or red fluorophores. A special liquid-crystal color filter is available for color imaging of histology slides. Click on the following links for available filters and objectives.

Nikon Inverted. This is an older model Nikon Diaphot 200 microscope with a Princeton Instruments MicroMax CCD camera and filter wheels for integrated operation via MetaMorph. Click on the following links for available filters and objectives.

Live Cell Chambers:

Warner PDMI-2 Open Perfusion Micro-Incubator. This is an open system that takes 35 mm coverglass-bottomed dishes. It can be used under static or perfusion conditions. It can be mounted on the Leica SP1/MP or Zeiss META confocals, the DeltaVision or DeltaVision RT microscopes, or the Nikon Inverted.

Bioptecs FCS2 Chamber. This is a closed perfusion chamber that requires that you culture cells on a special size coverglass. The coverglass with the cells is assembled with the other components of the chamber to create a closed system providing very uniform laminar flow conditions. It can be mounted on the DeltaVision, DeltaVision RT, or Nikon Inverted microscopes.

Image Processing Computers:

There are several computers available for image analysis. Which one you choose will depend on what you are trying to accomplish and what software you are comfortable using. Every Keck Center user has access to these computers at no charge for analyzing images collected with the Keck Center's microscopes.

1) A PC running MetaMorph, a very powerful image analysis program. It is often the best choice as it will extract many kinds of data from your images for export to Excel. MetaMorph can be automated for repetitive tasks and works well with image stacks. This computer also has Photoshop and ImageJ.

2) A wicked fast and powerful 64-bit windows PC running the DeltaVision SoftWorx program from Applied Precision. It is used to deconvolve image stacks. It also has 3D visualization and analysis tools from Imaris that can be used on the resulting deconvolved image sets.

3) A powerful PC computer running the DeltaVision SoftWorx program from Applied Precision. It is used to deconvolve image stacks, and has 3D visualization and analysis tools that can be used on the resulting deconvolved image sets.

EPSON Stylus Photo 1280 inkjet printer.

Contact Greg Martin at gvm@u.washington.edu for additional information. Suggestions and comments are always welcome!

Keck Microscopy Facility