Leica Confocal

Basic Procedures:

Starting the system

Setting up the software

Setting up the microscope

Collecting images

Image quality

Collecting images for 3D reconstruction

Saving images

Shutting down the system

Ending a confocal session


Advanced Procedures:

Live Imaging

Image Quality

collecting multiple labels

Differential Interference Contrast (DIC) microscopy

Collecting/Projecting a Z-Series

Collecting Sequential Scans

Collecting XZ Images

Reflection Imaging

FRAP



Features:

The Microscope

Objectives

Leica Confocal Software

Acousto-Optical Tunable Filter (AOTF)

Lasers

Panel Box

Electronic Zoom

The Confocal Pinhole

Fluorescence and Fluorescent filters



Troubleshooting

Keck Home Page