Leica Confocal Software
Select "Tools" from the top of the software menu and "Customize".
Drag and drop icons into the desktop to insert them into your profile. Note that we do not have all of the features (i.e.bit depth, scan rotation) so ask me if you have a question.
Drag any icons you don't want from the desktop and drop them into the trashcan in the customize menu.
To make this profile default for the display menu (i.e. ovl rgb, lut, etc) go to "Tools" and select "Options". Click on "Viewer Template" and "add active viewer". Give the profile a name. Select the profile from the list.
DO NOT use this button to change the objectives - you can damage the objectives if you don't lower the objective turret first. If it is in your profile please remove it.
Below is an example of a "customized" screen - click on some of the features for more information.
ITool has a number of useful features: merging images, arithmetic, splitting images, projecting part of a z-series, etc.
The basic menu will merge images collected in different files.
Extended Dynamic Range: start with 48% and 18%
Signal Gain and offset can be adjusted with the sliders under the signal button. This might be easier with multiple channels, or if there are problems with the panel box controls. These sliders are generally not as fine a control though as using the panel box knobs.
The boxes at the bottom of the window indicate the activated PMT's (channels).
Pan within the field of view when zoomed:
When the image is zoomed use these arrows to pan within the field of view. This does not replace the traverse on the microscope to change the field of view on the slide.
Use this scan button when setting up the microscope - adjusting gain and offset, zooming, focusing, setting up a z-series, etc.
When ready to collect the image, use one of the other two large scan buttons - Single Scan or Series.
Single Scan will collect one image, filtering out the noise with the number set with "Aver".
Series will collect the number of images set with the "Sect" button, filtering out the noise in each image with the number set with "Aver".
Scan continuously