Morphologic evaluation of several types of specimens is performed in the laboratory. Specimens reviewed include bone marrow biopsy and aspirate specimens, cases for hematopathology consultation, body fluids, and peripheral blood for Sezary cell evaluation.
Bone Marrow Biopsy and Aspirate
Bone marrow biopsy and aspirate specimens may be submitted for morphologic evaluation. For specimens collected at UWMC, a laboratory technologist will be available for bedside assistance with specimen collection during the hours of 8:30 and 3:30 Monday through Friday (excludes holidays). At other times a cart will be provided and stocked to facilitate specimen collection. Specimens submitted from outside the university may include stained or unstained long smears, touch preparations, fresh bone marrow aspirates, and bone marrow core biopsies fixed in formalin.
The laboratory provides hematopathology consultation services. When sending slides for consultation please include a report and any available clinical information and history. If it is anticipated that immunohistochemical stains will be needed, please send a paraffin block along with the case. Although a paraffin block is preferred, unstained paraffin sections on adhesive slides (charged or plus) may be sent instead. Please include the patient’s billing information with the request if the patient’s insurance is to be billed. If the patient billing information is not included the institution will be billed for services.
Cytological Examination of Body Fluids and Cerebrospinal fluid
Body fluid and CSF reviews are performed using a Wright-Giemsa stained (concentrated) cytocentrifuge preparation to evaluate these specimens for evidence of hematopoietic neoplasms. If clinical concern for a non hematopoietic malignancy exists, the specimen should be submitted to Cytology (Anatomic Pathology at Harborview Medical Center).
Sézary Cell Preparation
Sézary cells are peripheralized malignant T cells seen in some patients with mycosis fungoides. These cells may be detected by flow cytometry and can be identified by review of a Wright-Giemsa stained peripheral blood smear.
Last updated: 6/15/08