Petrie M. Rainey, M.D., Ph.D.
Professor, Laboratory Medicine
School of Medicine
University of Washington
- Department of Laboratory Medicine
- University of Washington
- Box 357110
- Seattle, WA 98195-7110
- Tel: (206)-598-6131
- Fax: (206)-598-6189
- Email: pmrainey@u.washington.edu
Qualifications
- B.S., 1968, University of North Carolina
- Ph.D., 1973, University of California, Berkeley
- M.D., 1980, University of North Carolina
- Fellowships: University of California, San Francisco; University of Regensburg, Germany
Research Interests
I have a variety of research interests related to therapeutic drugs, including development of analytical methods, investigations of pharmacokinetics, molecular and cellular investigations of mechanisms of action and resistance, and clinical studies. These have provided an assortment of projects for Laboratory Medicine residents and fellows, as well as undergraduate, graduate and medical students. Current research falls in three basic areas: Leishmaniasis and its treatment; the pharmacology of drugs used in the treatment of HIV infection; and various investigations related to Laboratory Medicine.
Leishmaniasis: The leishmaniases are a family of diseases caused by the parasitic protozoan Leishmania . We have been able to reproduce the entire life cycle in vitro and have used this system to study the regulation of gene expression during the transformations between amastigote and promastigote life stages. We are currently investigating the composition, mechanism of action and pharmacokinetics of the antileishmanial drugs, sodium stibogluconate and meglumine antimoniate.
References:
- Cruz A, Rainey PM, Herwaldt BL, Stagni G, Palacios R, Trujillo R, Saravia NG. Pharmacokinetics of Antimony in Children Treated for Leishmaniasis with Meglumine Antimoniate. J Infect Dis 2007; 195:602-608. Medline Abstract
- Roberts WL, McMurray WJ, Rainey PM. Characterization of the antimonial antileishmanial agent meglumine antimonate (glucantime). Antimicrob Agents Chemother 1998 May;42(5):1076-1082. (See model on right of 'Proposed Structure for Meglumine Antimonate (n=2)'). Medline Abstract
- Roberts WL, Berman JD, Rainey PM. In vitro antileishmanial properties of tri- and pentavalent antimonial preparations. Antimicrob Agents Chemother 1995 Jun;39(6):1234-1239. Medline Abstract
- Campbell SM, Rainey PM. Leishmania pifanoi: kinetics of messenger RNA expression during amastigote to promastigote transformation in vitro. Exp Parasitol 1993 Aug;77(1):1-12. Medline Abstract
AIDS pharmacology: Pharmacokinetics and pharmacodynamics of drugs used to treat HIV infection and associated opportunistic infections. We are currently studying interactions between anti-HIV drugs (reverse transcriptase inhibitors and protease inhibitors) and drugs used to treat substance abuse (methadone, LAAM, buprenorphine).
References:
- McCance-Katz EF, Moody DE, Morse GD, Friedland G, Pade P, Baker J, Alvanzo A, Smith P, Ogundele A, Jatlow P, and Rainey PM. Interactions Between Buprenorphine and Antiretrovirals I: Non-Nucleoside Reverse Transcriptase Inhibitors Efavirenz and Delavirdine. Clin Infect Dis 2006; 43(Suppl 4): S224-S234. Medline Abstract
- McCance-Katz EF, Moody DE, Smith PF, Morse GD, Friedland G, Pade P, Baker J, Alvanzo A, Jatlow P, and Rainey PM. Interactions Between Buprenorphine and Antiretrovirals II: Protease Inhibitors, Nelfinavir, Lopinavir/Ritonavir, or Ritonavir. Clin Infect Dis 2006; 43(Suppl 4): S235-S246. Medline Abstract
- McCance-Katz EF, Rainey PM, Smith P, Morse GD, Friedland G, Boyarsky B, Gourevitch M, and Jatlow P. Drug Interactions between Opioids and Antiretroviral Medications: Interaction between Methadone, LAAM and Delavirdine. Am J Addict 2006; 15(1):23-34. Medline Abstract
- Perlman DC, Segal Y, Rosenkranz S, Rainey PM, Remmel RP, Salomon N, Hafner R and Peloquin CA; ACTG 309 Team. The clinical pharmacokinetics of rifampin and ethambutol in HIV-infected persons with tuberculosis. Clin Infect Dis 2005; 41(11):1638-1647. Medline Abstract
- McCance-Katz EF, Rainey PM, Jatlow P, Friedland G. Methadone effects on zidovudine disposition (ACTG 262) J Acquir Immune Defic Syndr 1998;18(5):435-443. Medline Abstract
Laboratory Medicine: We carry out various investigations, both analytical and clinical, related to clinical chemistry, therapeutic drug monitoring and toxicology.
References:
- Pierce KM, Hoggard JC, Hope JL, Rainey PM, Hoofnagle AN, Jack RM, Wright BW, and Synovec RE. Fisher Ratio Method Applied to Third-Order Separation Data To Identify Significant Chemical Components of Metabolite Extracts. Anal Chem 2006; 78(14):5068-5075. Medline Abstract
- Hoofnagle AN, Laha TJ, Rainey PM, Sadrzadeh SMH. Specific detection of anabasine, nicotine and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. Am J Clin Path 2006; 126:880-887. Medline Abstract
- Roberts WL, Smith PT, Martin WJ, Rainey PM. Performance characteristics of three serum iron and total iron-binding capacity methods in acute iron overdose. Am J Clin Pathol 1999;112(5):657-64. Medline Abstract
- Rainey PM, Rogers KE, Roberts WL. Metabolite and matrix interference in phenytoin immunoassays. Clin Chem 1996 Oct;42(10):1645-1653. Medline Abstract
Professional Activities
Dr. Rainey is the Director of the Chemistry Division of the
Department of Laboratory Medicine and also serves as the Director of the Clinical Pathology Track of the Pathology Residency Training Program. He was the founding Chair of the
Point of Care Testing Division of the American Association for Clinical Chemistry ( AACC ).
He also helped to found the International Association for Therapeutic Drug Monitoring and Clinical Toxicology ( IATDMCT )
and served as the first Director of Education. Dr. Rainey is a past President of the Academy of Clinical Laboratory Physicians and Scientists
( ACLPS ) and also is active in the American College of Medical Toxicology
( ACMT ). He is on the editorial boards of the journals
Clinica Chimica Acta and Current Therapeutic Research.
Additional information is available at http://myprofile.cos.com/raineyp31 .
Last updated: 8/23/07