Tait Research Laboratory
Introduction
For the convenience of interested researchers, we provide selected protocols from our research laboratory. Protocols are provided "as is." Although we will endeavor to provide updates and corrections as needed, we are a small laboratory, and therefore we may be unable to reply to individual requests for advice, assistance, and troubleshooting.
Plasmids
Plasmids pET12a-PAPI (expresses human wild-type annexin V) and pJ128 (expresses the annexin V-128 protein) are available from the Addgene repository: http://www.addgene.org/pgvec1?f=c&cmd=showcol&colid=553
Laboratory Protocols for Annexin Research in downloadable PDF format:
- Determination of HYNIC-protein conjugation ratio by colorimetric assay with pNBA [34K pdf*]
- Preparation of tin-tricine reagent for Tc labeling of HYNIC-derivatized proteins [29K pdf*]
- Determination of binding affinity at low membrane occupancy by annexin V-RBC calcium titration assay) [19K pdf*]
- Preparation of Tin/Glucoheptonate/Gentisic Acid Reagent [57K pdf*]
- Reduction of Cysteine in Annexin V-128 [73K pdf*]
- 99mTc Labeling of Annexin V-128 with N terminal peptide chelation site [77K pdf*]
- Production of Annexin V-128 in E. coli [1.56M pdf*]
- Production of Annexin V (wild type) in E. coli [118K pdf*]
- Labeling of Annexin V with FITC [109K pdf*]
Last updated: 11/20/2008