Principles of Gene Regulation

Course Number: 
GENOME 551
Course Type: 
Keystone
Currently Offered: 
Yes
Credits: 
1.5
Quarter: 
Winter
Weeks: 
1-5
Meeting Time: 
T Th 1030-1150
Location: 
UW
Instructor (non-MCB Faculty): 
John Stamatoyannopoulos
Course Description: 

A detailed examination of the mechanisms of transcription and translation in prokaryotes and eukaryotes as determined by experimental genetics, molecular biology and biochemistry.

Course Web site: 

Sample Schedule:

Week Tues Thurs
  1

Promoter structure and promoter recognition in prokaryotes.

Structure and function of RNA polymerase.

Closed and open transcriptional complexes.

Genetic analysis of the lac operon.

  2

DNA looping and regulation of the ara operon.

Class I and II CRP-dependent promoters.

Attenuation at the trp operon.

Methods for determining where proteins bind on DNA. 

  3

Methods for determining where transcription initiates.

Methods for determining protein/protein interaction.

Promoter structure, enhancers, insulators and locus control regions.

Structure and function of RNA polymerase II and TAFs.

  4

Classes of transcription factors.

Co-activators and co-repressors.

Nucleosome structure.

ATP-dependent nucleosome remodeling factors.

  5

Chromatin modification by methylation, acetylation  and phosphorylation of histones.

The histone code.

Shine-Dalgano mediated translational initiation in prokaryotes.

Ribosome scanning mediated translational initiation in eukaryotes.

     

Areas of Interest: 
Gene Expression, Cell Cycle & Chromosome Biology
Areas of Interest: 
Genetics, Genomics & Evolution
Elective Area: 
Cancer Biology