cpilling

Student Profile

First Name: 
Carissa
Last Name: 
Pilling
[field_fname-formatted] [field_lname-formatted]
Primary Institution: 
FHCRC
Department/Division: 
other
Department/Division: 
other
MCB Entry Quarter: 
Autumn
MCB Entry Year: 
2011
E-Mail: 
Mail/Box #: 

357275

Phone: 
(206) 543-0253
Lab Information
Advisor: 
Lab Location: 
A2 111
Lab Phone #: 
(206) 667-4465
Research
Research Summary: 

 

The first human protein discovered to be a potent tumor driver was the tyrosine kinase Src. After over 40 years of research on Src we know that over expression rather then genetic mutation of Src results in its powerful tumor driver phenotype; however, we still do not understand how a cell maintains the proper amount of Src protein thereby preventing tumor formation.  The goal of my work in the Cooper lab is to identify and map the mechanism the ubiquitin proteasome system uses to control Src protein levels.  More specifically I am interested in understanding how the E3-ubiquitin ligase Cullin5 regulates Src protein levels.

We know that active Src protein levels go up when Cullin5 is knocked down in epithelial cells and this results in transformation of these cells.  My goals in the Cooper lab are 1) Identify the substrate adaptor Cullin5 uses to recognize Src 2) Identify the Degron directing Src to Cullin5 3) Determine if interactions between Cullin5 substrate adaptor proteins and Src are direct or indirect.  This will give us a better understanding the molecular switch that can be manipulated turning normal cell growth to the uncontrolled growth state know as cancer. 

Areas of Interest: 
Cancer Biology
Cell Signaling & Cell/Environment Interactions
Publications: 
24284072
Academic Information
Joint / Related Programs: 
Biotech Externship
TA/Out Reach
Class/Activity: 
Biochemistry 442
Quarter: 
Spring
Year: 
2013
Class/Activity: 
SEP
Quarter: 
Summer
Year: 
2013