We are using mouse embryonic stem cell approaches to introduce novel genetic mutations into the PKA regulatory system and investigating the major physiological functions of this signal transduction cascade. The mouse allows a full complement of genetic manipulations and in most instances provides a relevant model of human physiology, behavior, and disease. We use genetic techniques to produce tissue-specific activation or inactivation of PKA subunits and AKAPs (PKA scaffolding proteins). The lab has also developed a chemical-genetic approach that will allow drug dependent control of kinase activity in specific cell types in vivo.
Copyright © 2003-2013 Molecular & Cellular Biology Program, University of Washington
Fred Hutchison Cancer Research Center | University of Washington
Institute for Systems Biology | Seattle Biomed