rkbradle

Alternative splicing, the process by which a single gene can give rise to multiple, distinct protein isoforms, affects the vast majority of human genes. This mechanism enormously increases the complexity of eukaryotic genomes, and plays important roles in many human diseases.

We use genomics, sequence analysis, and molecular genetics to study the mechanistic origins and phenotypic consequences of alternative splicing and other RNA processing. A typical experiment might combine high-throughput sequencing to identify a molecular phenotype with minigene experiments to test specific biochemical predictions.

We also study transcriptional regulation, with a focus on the origins of genome-wide transcription factor binding.