We are a bioanalytical laboratory that develops separation science and mass spectrometric methods to solve biological problems of interest to us and our collaborators. Our primary tool is mass spectrometry and specifically Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). We are awaiting delivery of a Thermofinnigan LTQ-FT in April of 2004. In addition we have purchased an upgrade to the Bruker APEX III FTICRMs instrument located in our department that will allow electron capture dissociation (ECD) with infrared multi-photon dissociation (IRMPD) as well as collision induced dissociation of ions in quadrupoles located prior to the ICR cell. We are developing a novel form of parallel tandem mass spectrometer with Michrom Bioresources of Auburn, CA and testing a MALDI-ion trap-TOF-MS from Shimadzu Corp. We are applying our technologies in the fields of microbiology with Dr Samuel Miller at the UW, lycopene and prostate cancer with Dr Richard Richard van Breemen of UIC, androgen receptor binding proteins and prostate cancer with Dr Daniel Martin at the ISB, Acute respiratory distress syndrome with Dr Lynn Schnapp of the UW, and pediatric medulloblastomas with Dr Jim Olson of the FHCRC. More about these projects can be found on the Goodlett lab page.

Recent Publications:

1. S. Purvine, J.T. Eppel, E.C. Yi, D.R. Goodlett "Shotgun peptide collision induced dissociation on an electrospray ionization-time-of-flight mass spectrometer" Proteomics 2003, 3, 847.

2. A.G. Utleg, E.C. Yi, T. Xie, P. Shannon, J.T. White, D.R. Goodlett, L. Hood, B. Lin "Proteomic analysis of prostasomes" Prostate 2003, 56, 150.

3. J.A. Ranish, E.C. Yi, D.M. Leslie, S.O. Purvine, D.R. Goodlett, J. Eng, R. Aebersold "The study of macromolecular complexes by quantitative proteomics" Nat. Genetics 2003, 33, 349.

4. T. Guina, S.O. Purvine, E.C. Yi, J. Eng, D.R. Goodlett, R. Aebersold, S.I. Miller "Quantitative Proteomic Analysis Indicates Pseudomonas aeruginosa Produces A Quinolone Signal In Cystic Fibrosis Airways" Proc Natl Acad Sci U S A, 2003, 100, 2771.

5. Y. Sydorsky, D.J. Dilworth, E.C. Yi, D.R. Goodlett, J.D. Aitchison "Intersection of the kap123p-mediated nuclear import and ribosome export pathways" Mol Cell Biol. 2003, 23, 2042.

6. N.S. Baliga, M. Pan, Y.A. Goo, E.C. Yi, D.R. Goodlett, K. Dimitrov, P. Shannon, R. Aebersold, W.V. Ng, L. Hood. "Coordinate regulation of energy transduction modules in Halobacterium sp. analyzed by a global systems approach" Proc Natl Acad Sci U S A 2002, 99, 14913.

7. E.C. Yi, H. Lee, S.O. Purvine, R. Aebersold, D.R. Goodlett "Approaching complete peroxisome charcterization by gas-phase fractionation" Electrophoresis 2002, 23, 3205.

8. Y. Shiio, S. Donohoe, E.C. Yi, D.R. Goodlett, R. Aebersold, R.N. Eisenman "Quantitative Proteomic Analysis of Myc Oncoprotein Function" E.M.B.O. 2002, 21, 5088.

9. D.R. Goodlett, E.C. Yi "Proteomics without Polyacrylamide" Functional Integrative Genomics 2002, 2, 138.

10. S. Dramsi, M.P. Scheid, A. Maiti, P. Hojabrpour, X. Chen, K. Schubert, D.R. Goodlett, R. Aebersold, V. Duronio "Identification of a Novel Phosphorylation Site, Ser170, as a Regulator of Bad Pro-apoptotic Activity." J.Biol.Chem. 2001, 277, 6399.

11. D.R. Goodlett, J.D. Watts, P. von Haller, S. Donohoe, E. Yi, S. Purvine, R. Newitt, J.K. Eng, R. Aebersold "Differential Isotopic Esterification for Quantification and de novo Sequencing of Proteins" Rapid Commun Mass Spectrom 2001, 15, 1214.

12. P. von Haller, S. Donohoe, D.R. Goodlett, R. Aebersold, J.D. Watts, "Mass Spectrometric Characterization of Proteins Extracted from Jurkat T-Cell Detergent Resistant Membranes" Proteomics 2001, 1, 1010.

13. M.G. Katze, B. Kwieciszewski, D.R. Goodlett, C.M. Blakely, P. Nedderman, S-L Tan, R Aebersold "Ser2194 is a highly conserved Major Phosphoreylation Site of the Hepatitis C Virus Nonstructural protein NS5A" Virology 2000, 278, 501.

14. R. Aebersold and D.R. Goodlett "Proteomics and Mass Spectrometry" Chemical Reviews 2001, 101, 269.

15. D.R. Goodlett, R. Aebersold, J.D. Watts "Quantitative in vitro Kinase Reaction as a Guide for Phosphoprotein analysis by Mass Spectrometry" Rapid Commun Mass Spectrom. 2000, 14, 344.

16. D.R. Goodlett, J.E. Bruce, G.A. Anderson, B. Rist, L. Pasa-Tolic, O. Fiehn, R.D. Smith, R. Aebersold "Protein Identification with a Single Accurate Mass of a Cysteine-containing Peptide and Constrained Database Searching" Anal Chem. 2000, 72, 1112.

17. M. Heller, D.R. Goodlett, J.D. Watts, R. Aebersold "A comprehensive characterization of the T-cell antigen receptor complex composition by microcapillary liquid chromatography-tandem mass spectrometry" Electrophoresis 2000, 21, 2180.

18. S.A. Susin, H.K. Lorenzo, N. Zamzami, I. Marzo, G. Brothers, B. Snow, E. Jacotot, P. Costantini, N. Larochette, D.R. Goodlett, R. Aebersold, G. Pietu, M.-C. Prevost, D. Siderovski, J. Penninger, G. Kroemer "Molecular characterization of mitochondrial apoptosis-inducing factor." Nature 1999, 397, 441.

19. B. Gallis, G.L. Corthals, D.R. Goodlett, H. Ueba, F. Kim, S.R. Presnell, D. Figeys, D.G. Harrison, B.C. Berk, R. Aebersold, M. Corson "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002" J. Biol. Chem., 1999, 274, 30101.

20. D. Figeys, G.L. Corthals, B. Gallis, D.R. Goodlett, A. Ducret, M.A. Corson, R. Aebersold "Data-dependent modulation of solid-phase extraction capillary electrophoresis for the analysis of complex peptide and phosphopeptide mixtures by tandem mass spectrometry: application to endothelial nitric oxide synthase" Anal. Chem. 1999, 71, 2279.

21. N. Lee, D.R. Goodlett, H. Marquardt, D.E. Geraghty "HLA-E surface expression depends on binding of TAP-dependent peptides derived from certain HLA class I signal sequences"J. of Immunology 1998, 160, 4951.

22. D.R. Goodlett, P. Abuaf, T. Mukherjee, J.B. Crowther, N. Corkum, G. Goldstein "Peptide chiral purity determination: hydrolysis in deuterated acid, derivatization with Marfey's reagent and analysis using high-performance liquid chromatography-electrospray ionization-mass spectrometry"J.Chromatogr.A 1995,707, 233.

23. D.C. Gale, D.R. Goodlett, K.J. Light-Wahl, R.D. Smith "Observation of Duplex-DNA Noncovalent Complexes by electrospray Ionization-Mass Spectrometry" J. Am. Chem. Soc. 1995, 116, 6027.

24. D.R. Goodlett, R.R.O. Loo, J.A. Loo, J.H. Wahl, H.R. Udseth, R.D. Smith "A study of the Thermal Denaturation of Ribonuclease S by Electrospray Ionization Mass Spectrometry" J. Am. Soc. Mass Spectrom. 1995, 5, 614.

25. J.H. Wahl, D.R. Goodlett, H.R. Udseth, R.D. Smith "Use of small-diameter capillaries for increasing peptide and protein detection sensitivity in capillary electrophoresis-mass spectrometry" Electrophoresis 1993, 14, 448.

26. J.H. Wahl, D.R. Goodlett, H.R. Udseth, R.D. Smith Attomole level capillary electrophoresis mass spectrometric protein analysis using 5 um id capillaries. Anal. Chem. 1992, 64, 3194-3196.

27. D.R. Goodlett, D.G. Camp, C.C. Hardin, M. Corregan, R.D. Smith "Direct observation of a DNA quadruplex by electrospray ionization mass spectrometry" Biol. Mass Spectrom. 1993, 22 181.

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This page last updated: February 2, 2004