ACADEMIC BIO

• B.S., 1982, Auburn University, Alabama
• M.S., 1988, Auburn University, Alabama
• Ph.D., 1991, North Carolina State University

RESEARCH OVERVIEW

We are a bioanalytical laboratory that develops separation science and mass spectrometric methods to solve biological problems of interest to us and our collaborators. In general we seek to use mass spectrometry to extract the maximum amount of information with a minimum of fractionation prior to MS analysis, which we refer to as Indolent Driven Science. Our primary tool is mass spectrometry and specifically Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS). We have upgraded an older Bruker APEX III FTICRMs instrument to allow electron capture dissociation (ECD) with infrared multi-photon dissociation (IRMPD) as well as collision induced dissociation of ions in quadrupoles located prior to the ICR cell. Additionally, we have implemented a simple version of _d_ynamic _r_ange _e_xpansion _a_pplied to _m_ass _s_pectrometry (DREAMS) developed by the R.D. Smith laboratory on this instrument. We are currently pursuing data-independent analysis of complex mixtures using a protocol we refer to as Precursor Acquisition Independent from Ion Count (PAcIFIC; see Panchaud Anal Chem 2009) that is related to a type of data produced by a novel tandem mass spectrometer (see Wang RCMS 2007). Most recently we implemented an ion funnel on our Thermo LTQ-FT instrument (see Kelly et al. Mass Spectrom Rev 2009). Other workhorse instruments in the laboratory include a Waters SYNAPT, a Thermo LTQ-Velos and a Thermo LTQ-Orbitrap all fitted with Waters NanoAcquity HPLCs. We are applying these and other technologies with our collaborators who are in the fields of microbiology, prostate cancer, urinary tract disorders, HIV and fatigue, acute respiratory distress syndrome, and pediatric medulloblastomas. Finally, we are part of the University of Washington's Proteomics Resource **** (UWPR) consortium developed to aid proteomics research on campus. See Goodlett Lab Page.

RECENT SELECTED PUBLICATIONS

• Pan S, Goodlett DR et al. "Quantitative proteomics investigation of pancreatic intraepithelial neoplasia." Electrophoresis 30:7, 1132-1144 (2009).


• Nunn BL, Goodlett DR et al. "Deciphering diatom biochemical pathways via whole-cell proteomics." Aquatic Microbial Ecology 55:241-253 (2009).


• Wang X, Lundgren AD, Singh P, Goodlett DR, Plymate SR, Wu J., "An six-amino acid motif in the alpha3 domain of MICA is the cancer therapeutic target to inhibit shedding." Biochem Biophys Res Commun. 387:476-81 (Epub 2009 Jul 16).


• Panchaud A, Scherl A, Shaffer SA, von Haller PD, Kulasekara HD, Miller SI, Goodlett DR. "Precursor Acquisition Independent From Ion Count: How to Dive Deeper into the Proteomics Ocean." Anal Chem. (Epub ahead of print 2009 Jul 2).


• Coats SR, Jones JW, Do CT, Braham PH, Bainbridge BW, To TT, Goodlett DR, Ernst RK, Darveau RP. "Human Toll-like receptor 4 responses to P. gingivalis are regulated by lipid A 1- and 4'-phosphatase activities." Cell Microbiol. (Epub ahead of print 2009 Jul 13).