Mass
Spectrometry Center
University of Washington * School of Pharmacy
Department of Medicinal Chemistry
|
Instrument |
Year |
Ionization Source(s) & |
General Applications |
Image
|
|
Waters Micromass
Quattro Premier XE |
2006
|
± ESI & APCI (QHQ)MS/MS Tandem Quadrupole MS |
Automated LC/MS/MS quantification. Drug metabolism studies. Analysis of complex biological extracts. |  |
|
2006
|
± EI & CI (Q)MS Quadrupole MS |
Automated GC/MS quantitative and qualitative analysis. Drugs, metabolites, synthetic and natural products. |  |
|
|
2004
|
± MALDI TOF MS Time of Flight MS |
Characterization and identification of proteins by peptide mass mapping. PSD peptide sequencing. |  |
|
|
2004
|
± ESI & APCI (Q)MS Quadrupole MS |
Fully automated quantitative analysis of drugs and metabolites. |  |
|
|
2002 |
± ESI QH [FT(ICR)] MS/MS Fourier transform |
Identification & quantification of sample limited biologic materials by combined high resolution mass accuracy & high sensitivity |
 |
|
|
2002 |
± ESI (QH oa-TOF) MS/MS Tandem Hybrid MS |
Capillary LC/MS/MS, nano-flow & infusion analysis of proteolytic digests of biopolymers and peptides. |
 |
|
|
1999 |
± ESI & APCI (Q)MS Quadrupole MS |
Fully automated quantitative analysis of drugs and metabolites. |
 |
|
|
1995 |
± ESI & APCI (QHQ)MS/MS Tandem Quadrupole MS |
Automated LC/MS/MS quantification. Drug metabolism studies. Analysis of complex biological extracts. |
 |
|
|
2006 |
± ESI LTQ Orbitrap MS/MS Fourier transform |
Capillary LC/MS/MS of proteolytic digests of simple and complex mixtures. |
 |
|
|
2004 |
± ESI LTQ [FT(ICR)] MS/MS Fourier transform |
Capillary LC/MS/MS of proteolytic digests of simple and complex mixtures. |
 |
|
| *Goodlett Laboratory Proteomic Core instrumentation operated within the Center. | ||||
Click here for printer friendly version of the above instrument summary table (PDF)
| Waters
Micromass Quattro Premier XE Tandem Quadrupole Mass Spectrometer (QTQ)MS/MS |
|
|
Manufacturer:
|
Waters
Corporation, Milford, MA, USA
|
|
Mass
range:
|
3000 Dalton (Primary
& Secondary Beams)
|
|
Resolution:
|
Unit mass (Q1 &
Q2)
|
|
Interface:
|
Micromass Zspray™
Atmospheric Pressure Ionization (API) Source
|
|
Ionization
(±eV):
|
Electrospray
& Atmospheric Pressure chemical Ionization (ESI & APCI)
Atmospheric Pressure Photo Ionization (APPI) |
|
Inlet
Systems:
|
Liquid Chromatograph
(LC): |
|
Data
System:
|
Hardware:
IBM Pentium 4, 3.2 GHz, 1 Gbyte RAM
Software: Microsoft Windows XP, Micromass MassLynx® 4.1, MaxEnt® & BioLynx® |
|
Acquisition
Date:
|
2006
|
|
Funding:
|
Mass Spectrometry Center Equipment Budget
Office of the Dean of the School of Pharmacy Drug Metabolism, Transport, and Pharmacogenomics Research Program Department of Medicine/Metabolism, Endocrinology, Nutrition |
|
Predominant
Uses:
|
Drug, metabolite & conjugate studies (qualitative & automated quantification); tandem MS analysis of non-volatile and thermally-labile complex biological extracts and biopolymers; protein mass determination and digest analysis; peptide sequencing.
|
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Shimadzu
QP2010 Gas Chromatograph Quadrupole Mass Spectrometer (GC-QMS) |
|
|
Manufacturer:
|
Shimadzu
Scientific Instruments, Columbia, MD
|
|
Mass
range:
|
1.5 - 1024 Dalton
|
|
Resolution:
|
Unit mass
|
|
Ionization
(±eV):
|
Electron,
Chemical Ionization (EI, CI & NCI)
|
|
Inlet
Systems:
|
Gas
Chromatograph (GC): |
|
Data
System:
|
Hardware:
MPC, Pentium 4, 3.2 GHz, 1GB RAM
Software: Microsoft Windows XP, Shimadzu GCMS Solutions |
|
Acquisition
Date:
|
2005
|
|
Funding:
|
Mass Spectrometry Center Equipment Budget
|
|
Predominant
Uses:
|
Automated quantitative metabolic profiling in drug metabolism and drug interaction studies and the qualitative analysis of synthetic and natural products.
|
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Bruker
AutoFlex II Matrix-Assisted LASER Desorption Ionization -
Time of Flight Mass Spectrometer (MALDI-TOF)MS |
|
|
Manufacturer:
|
Bruker
Daltonics, Billerica, MA, USA
|
|
Mass
range:
|
Linear: > 132kDa
[Bovine Albumin (2MH+)]
Reflectron: > 6,000 Da |
|
Resolution:
|
Linear: > 4,000
(FWHM)* @ m/z 2,465.2 [ACTH (18-39)]
Reflectron: > 18,000 (FWHM)* @ m/z 3,147 (Somatostatin) |
|
Sensitivity:
|
Linear:
S/N>10:1 for 1 fmole at m/z 1,570.68 (Glu-Fib)
Reflectron: S/N>10:1 for 1 fmole at m/z 1,570.68 (Glu-Fib) |
|
Mass
Accuracy:
|
Linear:
< 100 ppm
Reflectron: < 10 ppm |
|
Ionization
(±eV):
|
Matrix
Assisted LASER Desorption (MALDI) - 337 nm Nitrogen laser
|
|
Detectors:
|
Two Micro
Channel Plates (MCP), Linear and Reflectron, 2 GHz time-to-digital
converter (TDC)
|
|
Inlet
Systems:
|
SCOUT
MTP Microtiter Plate Ion Source with Observation Optics
|
|
Data
System:
|
Hardware:
HP workstation XP 6000, 2 GHz dual-Xeon processor, 1GByte RAM
Software: Microsoft Windows 2000 (v5.0); Bruker Compass 1.0, FlexControl and Flex Analysis 2.0 and license, ClinProTools 2.0 GUI Integrated vacuum measurement and control unit |
|
Acquisition
Date:
|
2004
|
|
Funding:
|
Departments
of Biochemistry, Chemistry, Medicinal Chemistry, Periodontics and
Oral Biology, Medicine (Infectious Diseases)
Center for Ecogenetics and Environmental Health (CEEH) School of Pharmacy and its Drug Metabolism/Transport & Pharmacogenomics Research Program (DMTPR) University of Washington Mass Spectrometry Center |
|
Predominant
Uses:
|
Characterization of biopolymers (proteins, protein digests and oligonucleotides);
peptide sequencing using post source decay (PSD) techniques.
|
| *FWHM = full width at half height | |
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Waters Micromass Platform LCZ: Quadrupole Mass Spectrometer QMS | |
|
Manufacturer:
|
Waters
Corporation, Milford, MA, USA
|
|
Mass
range:
|
3000 Dalton
|
|
Resolution:
|
Unit mass
|
|
Interface:
|
Micromass Zspray™
Atmospheric Pressure Ionization (API) Source
|
|
Ionization
(±eV):
|
Electrospray
& Atmospheric Pressure chemical Ionization (ESI & APcI)
|
|
Inlet
Systems:
|
Liquid Chromatograph
(LC): |
|
Data
System:
|
Hardware:
Compaq Family 6 Model 7, x86, 384 Mbyte RAM
Software: Microsoft Windows NT 4.0, MassLynx® 3.4, MaxEnt® & BioLynx® |
|
Acquisition
Date:
|
2004
|
|
Funding:
|
|
|
Predominant
Uses:
|
Fully automated quantitative analysis of drugs and metabolites.
|
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Bruker
APEX Qe 47e: Fourier transform (Ion Cyclotron Resonance) Mass Spectrometer [FT(ICR)]MS |
|
|
Manufacturer:
|
Bruker
Daltonics, Billerica, MA, USA
|
| Magnet: | 160mm bore actively shielded
4.7 Tesla superconducting (Magnex Scientific Ltd., Oxford, UK) |
|
Mass
range:
|
> 66kDa (BSA specification)
|
|
Resolution:
|
> 450,000 (FWHM)*
|
|
Ionization
(±eV):
|
Electrospray
Ionization (ESI)
|
|
Inlet
Systems:
|
Infusion:
Cole Parmer Series 74900 Syringe pump (Cole Parmer Instrument Co., Vernon Hills, IL) Loop/Flow Injection Analysis (FIA) Syringe pump solvent delivery with Valco CI4W sample injector (500nl internal loop) (Valco Instruments Co., Houston, TX) Apollo Nanoflow Electrospray Ionization Source |
|
Data
System:
|
Hardware:
Dell WHL 1.7 GHz Xeon CPU, 512 Mbyte memory
Software: Microsoft Windows 2000, Hummingbird Exceed PC X Server, Bruker XMASS™(7.0.8) Bruker BioTools and RapiDeNovo™ (2.2) |
|
Acquisition
Date:
|
2002
|
|
Funding:
|
Mass
Spectrometry Center Equipment Budget
University of Washington Pharmacia (Gift) Merck Research Laboratories (Gift) Weyerhaeuser Company (Gift) The National Institutes of Health (NIH) |
|
Upgrades:
|
QH-hybrid
front end upgrade with CID, ECD, IRMPD, DREAMS
and reduced noise detection system and X-Y-Z Nanospray positioning
(2006)
|
|
Predominant
Uses:
|
Application of combined
high resolution, high accuracy mass measurement and high sensitivity
to the detection, identification and quantification of sample limited
biologic materials.
|
| *FWHM = full width at half height | |
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Waters
Micromass Q-Tof API-US: Quadrupole/Orthagonal acceleration Time-of-Flight Tandem Hybrid Mass Spectrometer (QHoa-TOF)MS/MS |
|
|
Manufacturer:
|
Waters
Corporation, Milford, MA
|
|
Mass
range:
|
Primary Beam (Q): 4000
Dalton (mass resolved mode)
32k Da (transmission mode – Rf only) Secondary Beam (oa -TOF): 32 k Da |
|
Resolution:
|
Primary Beam (Q): Unit
Mass
Secondary Beam (oa -TOF): > 10,000 @ FWHM* (V optics) > 17,500 @ FWHM (W optics) |
|
Ionization
(±eV):
|
Z-spray
Atmospheric Pressure Ionization (API) Source
Electrospray & Atmospheric Pressure chemical Ionization Nanoflow-Z Nebulized Pico Tip Electrospray Option with observation camera |
| Detectors: | Primary Beam: Electron
Multiplier (EM) Secondary Beam: Chevron dual micro channel plate (MCP) 3.6 GHz time-to-digital converter (TDC) ion counting |
|
Inlet
Systems:
|
Liquid
Chromatograph (LC):
Waters nanoACQUITY Ultra Performance LC™ (UPLC™) System (Waters Corp., Milford, MA) Infusion: Integral syringe pump solvent delivery (100nl - 2000nl min.-1) Loop/Flow Injection Analysis (FIA): LC solvent delivery (0.01ml – 40.00ul min.-1) with sample injection Syringe pump solvent delivery (100nl - 2000nl min.-1) with sample injection |
|
Sampling
System:
|
Autoinjector integral
to the Waters nanoACQUITY UPLC™
System
Valco CI4W sample injector (1ul internal loop) (Valco Instruments Co., Houston, TX) |
|
Data
System:
|
Hardware:
IBM IntelliStation M Pro, Pentium 4, 1.7GHz, 1Gbyte RAM
Software: Microsoft Windows XP, Micromass MassLynx 4.1, MaxEnt, BioLynx, ProteinLynx Global |
|
Acquisition
Date:
|
2002
|
|
Funding:
|
MS/DS: |
|
Upgrades:
|
Waters
nanoACQUITY UPLC™
System, MassLynx 4.1 (2006)
|
|
Predominant
Uses:
|
Data dependent capillary
LC/MS/MS and nano-flow infusion analysis of proteolytic digests
of biopolymers enabling database search identification and/or de
novo sequencing of proteins; the characterization of their post-translational
modifications and point mutations; the use of ICAT and other quantitative
methodologies.
|
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Agilent
Technologies LC/MSD Series 1100: Quadrupole Mass Spectrometer (QMS) |
|
|
Manufacturer:
|
Agilent
Technologies, Palo Alto, CA
|
|
Mass
range:
|
3000
Dalton
|
|
Resolution:
|
Unit
mass
|
|
API
Interface:
|
Orthogonal
Spray Ionization Source
|
|
Ionization
(±eV):
|
Electropray
(ES) &
Atmospheric Pressure Chemical Ionization (APcI) |
|
Inlet
Systems:
|
Liquid
Chromatograph (LC):
Agilent Technologies Series 1100 binary solvent delivery system Series 1100 UV-VIS Diode Array Detector (DAD) (Agilent Technologies, Palo Alto, CA) |
|
Sampling
System:
|
Agilent
Technologies 1100 Well-Plate Autosampler
|
|
Data
System:
|
Hardware:
Dell Dimension 5100C Pentium 4, 3GHz CPU, 1 Gbyte RAM
Software: Microsoft Windows XP, LC/MSD ChemStation 10.02 (rev A) |
|
Acquisition
Date:
|
1999
|
|
Funding:
|
Mass
Spectrometry Center Equipment Budget
U of W |
|
Upgrades:
|
LAN control, High Performance and Autosampler (2004)
Dell 5100C and Windows XP (2006) |
|
Predominant
Uses:
|
Fully
automated quantitative analyses
|
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Waters
Micromass Quattro II: Tandem Quadrupole Mass Spectrometer (QHQ) MS/MS |
|
|
Manufacturer:
|
Waters Corporation, Milford, MA, USA |
|
Mass
range:
|
4,000 Dalton (Primary & Secondary Beams) |
|
Resolution:
|
Unit mass (Q1 & Q2) |
|
Interface:
|
Micromass Zspray™ Atmospheric Pressure Ionization (API) Source |
|
Ionization
(±eV):
|
Electrospray & Atmospheric Pressure chemical Ionization (ESI & ApcI) |
| Ion Detection : | Daly detector after Q1 & Q2 |
|
Inlet
Systems:
|
Liquid Chromatograph
(LC): |
|
Sampling
System:
|
Shimadzu
SIL-10ADVP Auto injector (Shimadzu Scientific Instruments, Inc., Columbia, MD) Rheodyne 8125 or 7125 Manual Injectors (Rheodyne Inc., Cotati, CA) |
|
Data
System:
|
Hardware:
Hard Drives NW 1GHz Pentium III, 512 Mbyte RAM Software: Microsoft Windows XP, Micromass MassLynx® 4.0, MaxEnt® & BioLynx® |
|
Acquisition
Date:
|
1995 |
|
Funding:
|
MS/DS:
Pharmacia,
Inc. (Gift) |
|
Upgrades:
|
Zspray, NT
& Auto injector (1999) Windows XP and MassLynx® 4.0 (2006) |
|
Predominant
Uses:
|
Drug, metabolite & conjugate studies (qualitative & automated quantification); tandem MS analysis of non-volatile and thermally-labile complex biological extracts and biopolymers; protein mass determination and digest analysis; peptide sequencing. |
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Thermo
Linear Trap Quadrupole Fourier transform (Ion Cyclotron Resonance) Mass Spectrometer LTQ-FT MS/MS |
|
|
Manufacturer:
|
Thermo Electron Corp., San Jose, CA, USA |
| Magnet: | actively shielded 7.0 Tesla superconducting |
|
Mass
range:
|
≤ 2000 Da (normal mode), 4000 Da (extended mode) |
|
Resolution:
|
Unit
mass (LTQ MS) ≤ 500,000 (FWHM)* at m/z 400, typically 100,000 [FT(ICR) MS] |
|
Ionization
(±eV):
|
Electrospray Ionization (ESI) |
|
Inlet
Systems:
|
Liquid Chromatograph
(LC): |
|
Sampling
System:
|
MIchrom Endurance Bio-Cool Paradigm AS1 Autosampler (Michrom BioResources, Inc.) |
|
Data
System:
|
Hardware:
Dell Optiplex
GX270, Pentium 4, 2.8GHz, 1 Gbyte RAM Software: Microsoft Windows XP Professional, Excalibur 2.0 SR1™, LTQ-FT MS 2.0 RC6, Magic AutoSampler 2.0 & Paradigm MS4 2.0 |
|
Acquisition
Date:
|
2004 |
|
Funding:
|
The National Institutes of Health (NIH): NCRR 1 S10 RR17262-01 award entitled: "Fourier transforms-ion cyclotron-resonance mass spectrometer" |
|
Predominant
Uses:
|
Automated “shotgun” proteomic analysis - Combining nano-LC, data dependent MS/MS with high sensitivity (sub fmol on-column) and accurate mass measurement (2 ppm) for protein identifications in simple and complex protein mixtures. LC/MS/MS, label-free quantification, and accurate mass measurements. |
|
Click here for printer friendly version of this instrument's information (PDF) |
|
| Thermo
Linear Trap Quadrupole Fourier transform (Orbitrap) Mass Spectrometer LTQ-OT MS/MS |
|
|
Manufacturer:
|
Thermo Electron Corp., San Jose, CA, USA |
|
Mass
range:
|
≤ 2000 & 4000 Dalton (normal and extended modes, respectively) |
|
Resolution:
|
Unit
mass (LTQ MS) ≤ 100,000 (FWHM)* at m/z 400, typically 60,000 (OT MS) |
|
Ionization
(±eV):
|
Electrospray Ionization (ESI) |
| Ion Detection: | Electron multipliers
(LTQ MS) Fourier transform image current (OT MS) |
|
Inlet
Systems:
|
Liquid
Chromatograph (LC):
Waters nanoACQUITY Ultra Performance LC™ (UPLC™) System (Waters Corp., Milford, MA) Infusion: Integral syringe pump solvent delivery (100nl - 2000nl min.-1) Loop/Flow Injection Analysis (FIA): LC solvent delivery (0.01ml – 40.00ul min.-1) with sample injection Syringe pump solvent delivery (100nl - 2000nl min.-1) with sample injection |
|
Sampling
System:
|
Autoinjector integral
to the Waters nanoACQUITY UPLC™
System
Valco CI4W sample injector (1ul internal loop) (Valco Instruments Co., Houston, TX) |
|
Data
System:
|
Hardware:
Dell Optiplex
GX52, Pentium 4, 3.2GHz, 1 Gbyte RAM Software: Microsoft Windows XP Professional, Excalibur 2.0 SR1™, Magic AutoSampler 2.0 & Paradigm MS4 2.0 |
|
Acquisition
Date:
|
2006 |
|
Funding:
|
The National Institutes of Health (NIH): NCRR 1 S10 RR17262-01 award entitled: “Fourier transforms-ion cyclotron-resonance mass spectrometer” |
|
Predominant
Uses:
|
Automated “shotgun” proteomic analysis - Combining nano-LC, data dependent MS/MS with high sensitivity (sub fmol on-column) and accurate mass measurement (2 ppm) for protein identifications in simple and complex protein mixtures. LC/MS/MS, label-free quantification, and accurate mass measurements. |
| *FWHM = full width at half height | |
|
Click here for printer friendly version of this instrument's information (PDF) |
|
 |
Instruments
| Services | Contacts
| Rates
Sample Submission | Links | Home Department of Medicinal Chemistry School of Pharmacy | University of Washington For more information about the Mass Spectrometry Center, contact Bill Howald For comments or suggestions on this web site, contact Jeanine Kanov Last updated: October 8, 2007 |
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