Research in the Emery laboratory is focused on basic and translational research in the field of hematopoietic stem cell gene therapy. Area 1: The identification, characterization, and application of chromatin insulators in order to improve the efficacy and safety of recombinant virus vectors. This includes in part the use of genetic screens and informatics to identify potential insulators, the study of insulator activity on chromatin structure, and the development of novel tumor formation assays to study vector-related genotoxicity. Area 2: The development of lineage-specific recombinant viral vectors designed for the regulated, ex vivo expansion of red blood cells and megakaryocytes, and the testing of these vectors in mice. Area 3: The regulated expansion of cord blood stem/progenitor cells. Area 4: The use of large animal models for assessing viral vector-transduced hematopoietic stem/progenitor cells.
Emery DW, Holley K, Sachs DH. 1999. Enhancement of swine progenitor chimerism in mixed swine/human bone marrow cultures with swine cytokines. Exp Hematol 27:1330-1337. Emery DW, Andrews RG, Papayannopoulou T. 2000. Differences among nonhuman primates in susceptibility to bone marrow progenitor transduction with retrovirus vectors. Gene Ther 7:359-367. Emery DW, Yannaki E, Tubb J, Stamatoyannopoulos G. 2000. A chromatin insulator protects retrovirus vectors from position effects. Proc Natl Acad Sci 97:9150-9155. Jin L, Zeng H, Chien S, Otto KG, Richard RE, Emery DW, Blau CA. 2000. In vivo selection using a cell growth switch. Nat Genet 26:64-66. Sonntag K-C, Emery DW, Yasumoto A, Haller G, Germana S, Sablinski T, Shimizu A, Yamada K, Shimada H, Arn S, Sachs DH, LeGuern C. 2001. Tolerance to solid organ transplants through transfer of MHC class II genes. J Clin Invest 107:65-107. Emery DW, Tubb J, Nishino Y, Nishino T, Otto KG, Stamatoyannopoulos G, Blau CA. 2005. Selection with a regulated cell growth switch increases the likelihood of expression for a linked gamma-globin gene. Blood Cells Mol Dis 34:235-247. Li CL, Xiong D, Stamatoyannopoulos G, Emery DW. 2009. Genomic and functional assays demonstrate reduced gammaretroviral vector genotoxicity associated with use of the cHS4 chromatin insulator. Mol Ther 17:716-724. PMID: 19240697 Groth AC, Emery DW. 2010. A functional screen for regulatory elements that improve retroviral vector gene expression. Blood Cells Mol Dis 45:343-50. PMID: 20846887 Aker M, Bomsztyk K, Emery DW. 2010. Poly(ADP-ribose) polymerase-1 (PARP-1) contributes to the barrier function of a vertebrate chromatin insulator. J Biol Chem 285:37589-37597. PMID: 20876582 Okazuka K, Beard BC, Emery DW, Schwarzwaelder K, Spector MR, Sale GE, von Kalle C, Kiem HP, Blau CA. 2011. Long-term regulation of genetically modified primary hematopoietic cells in dogs. Mol Ther 19:1287-1294. PMID: 21326218 Liu M, Li CL, Stamatoyannopoulos G, Dorschner MO, Humbert R, Stamatoyannopoulos JA, Emery DW. 2012. Gammaretroviral vector integration occurs overwhelmingly within and near DNase hypersensitive sites. Hum Gene Ther 23:231-237. PMID: 21981728. Emery DW. 2011. The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors. Hum Gene Ther 22:761-774. PMID: 21247248