Research Program:

Our laboratory is interested in developing simple genetic strategies in yeast to analyze protein functions. These strategies include assays that analyze RNA, DNA, proteins, and small molecules. In one RNA-based application, we are developing a lariat RNA detection assay for the genomewide analysis of splicing and the annotation of genes. In DNA-based approaches, we are analyzing chromosome conformation in yeast and identifying proteins that function in recombination. For protein technologies, we are developing methods for the identification of protein targets of ubiquitin ligases and for the display of proteins in an array format. At the level of small molecules, we are using capillary electrophoresis to analyze the yeast metabolome. Our laboratory is also part of an NCRR Resource Center that is focused on the identification and characterization of protein complexes of yeast.

Investigator:  Dr. Fields is a Professor of Genome Sciences and Medicine (Medical Genetics).

Representative Publications:

LaCount, D.J., Vignali, M., Chettier, R., Phansalkar, A., Bell, R., Hesselberth, J., Schoenfeld, L.W., Ota, I., Sahasrabuhde, S., Kurschner, C., Fields, S., and Hughes, R. (2005) A protein interaction network of the malaria parasite Plasmodium falciparum.  Nature 438:103-107.

Kaeberlein, M., Powers, R.W. III, Steffen, K.K., Westman, E.A., Hu, D., Dang, N., Kerr, E.O., Kirkland, K.T., Fields, S., and Kennedy, B.K. (2005) Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients.  Science 310: 1193-1196.

Miller, J.P., Lo, R.S., Ben-Hur, A., Desmarais, C., Stagljar, I., Noble, W.S. and Fields, S. (2005) Large-scale identification of yeast integral membrane protein interactions.  Proceedings of the National Academy of Sciences U.S.A. 102: 12123-12128.

Kaeberlein, M., Hu, D., Kerr, E.O., Tsuchiya, M., Westman, E.A., Dang, N., Fields, S. and Kennedy, B.K. (2005) Increased life span due to calorie restriction in respiratory-deficient yeast. PLoS Genetics 1: e69: 614-621.

Brown, V., Brown, R.A., Ozinsky, A., Hesselberth, J.R. and Fields, S. (2006) Binding specificity of Toll-like receptor cytoplasmic domains. European Journal of Immunology 36: 742-753.

Powers, R.W. III, Kaeberlein, M., Caldwell, S.D., Kennedy, B.K. and Fields, S. (2006) Extension of chronological life span in yeast by decreased TOR pathway signaling. Genes and Development 20: 174-184.

Jin, F.,Hazbun, T., Michaud, G.A., Salcius, M., Predki, P.F., Fields S. and J. Huang (2006) A pooling-deconvolution strategy for biological network elucidation.  Nature Methods 3: 183-189.

Hesselberth, J.R., Miller, J.P., Golob, A., Stajich, J.E., Michaud, G.A. and Fields, S. (2006) Comparative analysis of Saccharomyces cerevisiae WW domains and their interacting proteins.  Genome Biology 7: R30.1-R30.15.

Zhang, Z., Hesselberth, J.R. and Fields, S. (2007) Genomewide identification of spliced introns using a tiling microarray.  Genome Research 17: 503-509.

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