Sakeneh Zraika, Ph.D.
Metabolism, Endocrinology and Nutrition
Research Assistant Professor of Medicine
Investigating mechanisms of pancreatic beta-cell dysfunction and death in type 2 diabetes. Use of rodent models to study impairment of insulin secretion in the setting of elevated glucose and fat. Emphasis on oxidative stress and derangements in insulin exocytosis. Study of novel aspects of islet amyloid formation, including degradation mechanisms.
Zraika S, Dunlop M, Proietto J, Andrikopoulos S: The hexosamine biosynthesis pathway regulates insulin secretion via protein glycosylation in mouse islets. Arch Biochem Biophys 405:275-279, 2002.
Zraika S, Dunlop M, Proietto J, Andrikopoulos S: Effects of free fatty acids on insulin secretion in obesity. Obes Rev 3:103-112, 2002.
Zraika S, Dunlop ME, Proietto J, Andrikopoulos S: Elevated SNAP-25 is associated with fatty-acid induced impairment of mouse islet function. Biochem Biophys Res Comm 317:472-477, 2004.
Kooptiwut S, Kebede M, Zraika S, Visinoni S, Aston-Mourney K, Favaloro J, Tikellis C, Thomas MC, Forbes JM, Cooper ME, Dunlop M, Proietto J, Andrikopoulos S: High glucose-induced impairment in insulin secretion is associated with reduction in islet glucokinase in a mouse model of susceptibility to islet dysfunction. J Molec Endocrin 35:39-48, 2005.
Zraika S, Aston-Mourney K, Kebede M, Laybutt DR, Dunlop ME, Proietto J, Andrikopoulos S: The influence of genetic background on the induction of oxidative stress and impaired insulin secretion in mouse islets. Diabetologia 49:1254-1263, 2006.
Zraika S, Hull RL, Udayasankar J, Clark A, Utzschneider KM, Tong J, Gerchman F, Kahn S.E: Identification of the amyloid-degrading enzyme, neprilysin, in mouse islets and potential role in islet amyloidogenesis. Diabetes 56:304-310, 2007.
Zraika S, Hull RL, Udayasankar J, Utzschneider KM, Tong J, Gerchman F, Kahn SE: Glucose- and time-dependence of islet amyloid formation in vitro. Biochem Biophys Res Comm 354:234-239, 2007.
Hull RL, Zraika S, Udayasankar J, Kisilevsky R, Szarek WA, Wight TN, Kahn SE: Inhibition of glycosaminoglycan synthesis and protein glycosylation with WAS-406 and azaserine result in reduced islet amyloid formation in vitro. Am J Physiol: Cell Physiology 293: C1586-1593, 2007.
Aston-Mourney K, Wong N, Kebede M, Zraika S, Balmer L, McMahon JM, Fam BC, Favaloro J, Proietto J, Morahan G, Andrikopoulos S: Increased nicotinamide nucleotide transhydrogenase levels predispose to insulin hypersecretion in a mouse strain susceptible to diabetes. Diabetologia 50:2476-2485, 2007.
Zraika S, Hull RL, Udayasankar J, Aston-Mourney K, Subramanian SL, Kisilevsky R, Szarek WA, Kahn SE: Oxidative stress is induced by islet amyloid formation and time-dependently mediates amyloid-induced beta cell apoptosis. Diabetologia 52:626-635, 2009.
Hull RL, Zraika S, Udayasankar J, Aston-Mourney K, Subramanian SL, Kahn SE: Amyloid formation in human IAPP transgenic mouse islets and pancreas, and human pancreas, is not associated with endoplasmic reticulum stress. Diabetologia 52:1102-1111, 2009.
Kahn SE, Zraika S, Utzschneider KM, Hull RL: The beta cell lesion in type 2 diabetes: there has to be a primary functional abnormality. Diabetologia 52:1003-1012, 2009.
Zraika S, Hull RL, Verchere CB, Clark A, Potter KJ, Fraser PE, Raleigh DP, Kahn SE: Toxic oligomers and islet beta cell death: guilty by association or convicted by circumstantial evidence? Diabetologia 53:1046-1056, 2010.
Zraika S, Aston-Mourney K, Marek P, Hull RL, Green PS, Udayasankar J, Subramanian SL, Raleigh DP, Kahn SE: Neprilysin impedes islet amyloid formation by inhibition of fibril formation rather than peptide degradation. J Biol Chem 285:18177-18183, 2010.