Josephine 'Josie' R. Chandler
Research Assistant Professor
Phone: (206) 221-2850
Office Location: Health Sciences K-359
Campus Box: 357735
Dr. Chandler is a Research Assistant Professor in Dr. Greenberg's laboratory. She received a B.S. in microbiology from the University of Iowa and a Ph.D. in Microbiology from the University of Minnesota. Her postdoctoral work was with Dr. E. Pete Greenberg at the University of Washington where she studied cell-cell communication in the non-pathogenic bacterium Burkholderia thailandensis. In the fall of 2013 Dr. Chandler will be transitioning to a faculty position in the Molecular Biosciences Department at the University of Kansas.
Dr. Chandler's research is focused on understanding how bacteria communicate and cooperate with each other to carry out complex group behaviors. She primarily studies a cell-cell communication system in bacteria called quorum sensing. Most quorum sensing systems become activated when the bacterial population reaches a critical cell density. Quorum sensing commonly controls production of extracellular factors, such as toxins, exoenzymes, and components involved in self-structured biofilm-like communities. Production of extracellular factors often involves cooperation because the factors can be shared among all of the members of a population. Much is known about the molecular biology of quorum sensing, but our understanding of its role in promoting group or cooperative activities remains obscure. Using Burkholderia thailandensis as a model, Dr. Chandler developed laboratory systems to understand how quorum sensing and cooperation are important for 1) competition between bacteria using antibiotics and 2) formation of self-structured communities. This work will promote our ability to create, maintain and manipulate cooperating bacterial communities that carry out important biological functions. In addition, bacteria serve as an excellent model for understanding the evolution of sociality.
Dr. Chandler's interests in the role of antibiotics in bacterial competition can be found here (http://beacon-center.org/blog/2013/04/22/bacterial-warfare-using-antibiotics-and-communication/)
Dr. Chandler is currently affiliated with Pete Greenberg at the University of Washington, whose research webpage can be found here (http://depts.washington.edu/epglab/)
Chandler JR, T Truong, PM Silva, M Radey, MA Jacobs, EH Sims, MR Seyedsayamdost, G Carr, J Clardy and EP Greenberg. 2012. Bactobolin resistance is conferred by mutations in the L2 ribosomal protein. mBio 3:e00499.
Goo E, CD Majerczyk, JH An, JR Chandler, Y-S Seo, H Ham, H Kim, B Lee, MS Jang, EP Greenberg and I Hwang. 2012. Quorum sensing, cooperativity and anticipation of stationary phase stress. Proc Natl Acad Sci USA 109:19775.
Chandler JR, S Heilmann, JE Mittler and EP Greenberg. 2012. Acyl-homoserine lactone- dependent eavesdropping promotes competition in a laboratory co-culture model. ISME J 6:2219.
Carr G, MR Seyedsayamdost, JR Chandler, EP Greenberg and J Clardy. 2011. Sources of diversity in bactobolin biosynthesis by Burkholderia thailandensis E264. Org Lett 13:3048.
Seyedsayamdost MR, JR Chandler, JA Blodgett, PS Lima, BA Duerkop, K Oinuma, EP Greenberg and J Clardy. 2010. Quorum-sensing-regulated bactobolin production by Burkholderia thailandensis E264. Org Lett 12:716.
Chandler JR, BA Duerkop, A Hinz, TE West, JP Herman, MEA Churchill, SJ Skerrett and EP Greenberg. 2009. A mutational analysis of Burkholderia thailandensis quorum sensing and self- aggregation. J Bacteriol 191:5901.
Duerkop BA, J Varga, JR Chandler, SB Peterson, JP Herman, MEA Churchill, MR Parsek, WC Nierman and EP Greenberg. 2009. Quorum-sensing control of antibiotic synthesis in Burkholderia thailandensis. J Bacteriol 191:3909.
Mattmann ME, GD Geske, GA Worzalla, JR Chandler, KJ Sappington, EP Greenberg and HE Blackwell. 2008. Synthetic ligands that activate and inhibit a quorum-sensing regulator in Pseudomonas aeruginosa. Bioorg Med Chem Lett 15:3072.