Research:
Dr. Katze is Professor of Microbiology and Associate Director and Core Staff Scientist at the Washington National Primate Research Center. He received his Ph.D. from Hahnemann Medical College and was a postdoctoral fellow at the University of Uppsala in Sweden as part of a fellowship with the European Molecular Biology Organization. Prior to joining the faculty at the University of Washington, Dr. Katze conducted research in molecular biology and virology at the Memorial Sloan-Kettering Cancer Center in New York City.
Research in the Katze lab is focused on virus-host interactions. In particular, the lab studies the varied and complex mechanisms used by viruses to avoid the interferon-mediated antiviral response and the clever ways by which viruses hijack the cellular protein synthesizing machinery. The lab is a leader in using the technologies of functional genomics, including DNA microarrays and proteomics, to study the wide constellation of changes in cellular gene expression and protein production that occur in response to virus infection. These technologies are used to analyze a broad range of experimental systems, including those focused on hepatitis C virus (HCV), influenza virus, Ebola virus, and simian immunodeficiency virus (SIV). Additional studies are aimed at profiling patterns of gene expression in serial liver biopsies obtained from patients who have undergone liver transplantation due to HCV-associated liver disease.
The lab is also working to develop a nationwide resource to facilitate the use of genomic technologies in nonhuman primate research, with particular emphasis on the rhesus macaque. The aim in establishing this resource is to provide the research community with the tools necessary to gain insight into the evolutionary relationship between human and macaque genomes and to better use the macaque as a model for reproduction, development, infection, and disease. Current work is focused on the construction of rhesus macaque cDNA libraries, large-scale expressed sequence tag (EST) sequencing of library clones, and the construction of oligonucleotide microarrays.
Selected Publications:
Jacobs, J.M., D.L. Diamond, E.Y. Chan, M.A. Gritsenko, W. Qian, M. Stastna, T. Baas, D.G. Camp II, R.L. Carithers Jr., R.D. Smith, and M.G. Katze. 2005. Proteome analysis of liver cells expressing a full-length hepatitis C virus (HCV) replicon and biopsies of post-transplanted liver from HCV-infected patients. J. Virol. 79:7558-7569.
Ladiges W.C., S.E. Knoblaugh, J.F. Morton, M.J. Korth, B.L. Sopher, C.R. Baskin, A. MacAuley, A.G. Goodman, R.C. LeBoeuf, and M.G. Katze. 2005. Pancreatic β-cell failure in mice with a deletion mutation of the endoplasmic reticulum molecular chaperone gene P58IPK. Diabetes. 54:1071-1081.
Baskin, C.R., A. García-Sastre, T.M. Tumpey, H. Bielefeldt-Ohmann, V.S. Carter, E. Nistal-Villán, and M.G. Katze. 2004. Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestrina). J. Virol. 78:10420-10432.
Coito, C., D.L. Diamond, P. Neddermann, M.J. Korth, and M.G. Katze. 2004. High-throughput screening of the yeast kinome: identification of human serine/theronine kinases that phosphorylate the hepatitis C virus NS5A protein. J. Virol. 78:3502-3513.
Kash J.C., C.F. Basler, A. García-Sastre, V. Carter, R. Billharz, D.E. Swayne, R.M. Przygodzki, J.K. Taubenberger, M.G. Katze, and T.M. Tumpey. 2004. Global host immune response: pathogenesis and transcriptional profiling of type A influenza viruses expressing the hemagglutinin and neuraminidase genes from the 1918 pandemic virus. J. Virol. 78:9499-9511.
Geiss, G.K., V.S. Carter, Y. He, B.K. Kwieciszewski, T. Holzman, M.J. Korth, C.A. Lazaro, N. Fausto, R.E. Bumgarner, and M.G. Katze. 2003. Gene expression profiling of the cellular transcriptional network regulated by alpha/beta interferon and its partial attenuation by the hepatitis C virus nonstructural 5A protein. J. Virol. 77:6367-6375.
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