Assistant Professor of Microbiology
Office Location: Health Sciences, J-137a
Campus Box: 357735
Dr. Mougous performed his graduate studies at the University of California at Berkeley in the laboratory of Dr. Carolyn Bertozzi. His thesis focused on the biosynthesis and role in virulence of sulfated glycolipids produced by Mycobacterium tuberculosis. As a postdoctoral fellow in the laboratory of Dr. John Mekalanos at Harvard Medical School, Dr. Mougous studied protein secretion in Pseudomonas aeruginosa.
A major focus of the Mougous lab is to understand, from both mechanistic and functional perspectives, the type VI protein secretion system (T6SS). This pathway, which is present in hundreds of Gram-negative bacteria, is remarkable in that it can target its substrates–some of which are toxins–to eukaryotic or prokaryotic cells. Current projects in our lab aim to identify novel T6SS substrates and characterize their mechanism-of-action, develop a structure-function model for the T6SS, define the steps leading to activation of the system, and to investigate the impact of the system on bacterial pathogenesis and microbial ecology. The lab employs a diverse toolkit that ranges from animal models of disease to protein crystallography.
LeRoux M, De Leon J, Kuwada NJ, Russell AB, Pinto-Santini D, Hood RD, Agnello DM, Robertson SM, Wiggins PA, Mougous JD(2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. PNAS 10.1073/pnas.1213963109.
Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD (2012) Structure of a peptidoglycan amidase effector targeted to gram-negative bacteria by the type VI secretion system. Cell Reports 10.1016/j.celrep.2012.05.016
Russell AB, Singh P, Brittnacher M, Bui NK, Hood RD, Carl MA, Agnello DM, Schwarz S, Goodlett DR, Vollmer W, Mougous JD(2012) A widespread bacterial type VI secretion effector superfamily identified using a heurisitic approach. Cell Host and Microbe 11(5): 539-549.
Silverman JM, Austin LS, Hsu F, Hicks KG, Hood RD, Mougous JD (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Molecular Microbiology 82(5): 1277-90.
Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475(7356): 343-7.
Ballister ER, Lai AH, Zuckermann RN, Cheng Y, Mougous JD (2008) In vitro self-assembly of tailorable nanotubes from a simple protein building block. PNAS 105(10): 3733-8.