| Enzyme | Exp. Date |
Notes |
| AciI | 6/09 | |
| AflIII | rec. 1/07 |
|
| AgeI-HF | 5/12 | |
| AhdI | 8/09 | |
| ApaI | 6/09 | |
| ApoI | 11/10 | |
| AscI | 10/11 | |
| AvrII | 1/11 | |
| BamHI | 11/09 | |
| BbvCI | 2/10 | |
| BglI | rec. 11/05 |
|
| BglII | 1/11 | |
| BlnI | rec. 6/09 | aka AvrII, from Roche |
| BlpI | 6/12 | |
| BmgBI | 2/10 | |
| BsaAI | 8/12 | |
| BsaBI | 10/09 | cuts at 60 degrees, dam and cpg sensitive |
| BseRI |
1/10 | |
| BsiWI | 2/09 | |
| BsmI | rec. 12/05 | |
| BspHI | 3/10 | |
| BsrBI | 1/11 | |
| BsrGI | rec. 7/06 |
|
| BssHII | 10/08 | |
| BssSI | 4/11 | |
| BstBI | 10/08 | |
| BstXI | 12/10 | |
| Bsu36I | rec. 8/06 |
|
| ClaI | rec. 10/06 |
prok. methylation sensitive |
| DpnI | 11/12 | |
| DraI | 11/08 | |
| DrdI | 1/10 | |
| EagI | 11/11 | |
| EarI | 4/09 | |
| EcoNI | 1/10 | |
| EcoRI | 1/12 | |
| EcoRV | 11/11 | |
| EcoICRI | rec. 2/08 |
from Promega |
| FseI | 7/09 | crap enzyme, store -80 |
| FspaI | 2/14 | from Fermentas, uses buffer 0, cpg sensitive |
| FspI | 4/09 | |
| HincII | rec. 11/05 | |
| HindIII | 1/09 | from Roche |
| HindIII | 8/12 |
|
| HpaI | rec. 3/06 |
|
| KpnI | 1/11 | |
| MfeI | 2/11 | |
| MluI | 3/12 | |
| MscI | 3/12 | |
| MslI | 8/09 | |
| NaeI | 8/11 | |
| NarI | 2/11 | |
| NcoI | rec. 11/05 | |
| NdeI | 08/12 | |
| NheI |
4/12 |
|
| NotI | 5/12 | |
| NotI-HF | 5/12 | |
| PacI | 8/12 | |
| PciI | 3/11 | |
| PflFI | 10/08 | |
| PflMI | 9/10 | |
| PshaI | 1/10, 8/12 |
|
| PsiI | 9/09 | |
| PstI | rec. 3/06 |
|
| PvuI | 6/12 | |
| PvuII | 2/12 | |
| RsrII | 2/10 | cuts poorly, add DTT if buffer is old |
| SacI | 10/11 | |
| SacII | 7/09 | |
| SalI | 11/11 | |
| SalI-HF | 3/12 | |
| SanDI | rec. 10/07 | from Stratagene |
| ScaI | 11/10 | |
| SexAI | rec. 11/05 |
|
| SfiI | rec. 7/06 |
|
| SfoI | 8/08 | cuts poorly |
| SgrAI | 12/09, 4/09 |
|
| SmaI | 9/10 | |
| SnaBI | rec. 1/07 |
|
| SpeI | 4/12 | |
| StuI | 10/08 | |
| SwaI | 12/08 | |
| Tth111I | 3/11 | |
| XbaI | 3/12 | prok. methylation sensitive |
| XcmI | rec. 11/05 |
not good for cloning |
| XhoI | 3/12 | |
| XmaI | rec. 11/05 |
|
| XmnI | 11/11 | |
| ZraI | 11/09 |
Restriction Enzymes
