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Wednesday, April 17, 2013 - 3:15pm

 

Flipping the Switch: The Neurobiology of Addiction
May 14, 2013 - 9am - 5pm

 

Click Here to register for the 2013 CIN Symposium:

Flipping the Switch: the Neurobiology of Addiction.
 

Free registration required

 

 

Location: Waterfront Activities Center, University of Washington, Seattle

Featured Speakers:
David Belin, INSERM, U Poiters
David Redish, Neuroscience, University of Minnesota
Catherine Winstanley, Psychology, University of British Columbia
 
Additional Speakers: 
Charles Chavkin, Pharmacology, UW
Jeremy Clark, Psychiatry, UW
Susan Ferguson, Seattle Children's Research Institute
Paul Phillips, Psychiatry, UW

 


Thursday, July 26, 2012 - 8:58am

Wambura Fobbs was just awarded a predoctoral fellow position on the UW Neurobiology Training Grant for three years. She was also selected as a Neuroscience Scholar via the Society for Neuroscience, Neuroscience Scholars Program (http://www.sfn.org/index.aspx?pagename=NeuroscienceScholars_Main) which is designed to enhance career development and professional networking opportunities for underrepresented students in neuroscience.  Congratulations Wambura!


Thursday, May 17, 2012 - 4:47pm
Thomas Coleman will present his work during the poster presentation of the Mary Gates Undergraduate Research Symposium.  Thomas' abstract is reprinted below.
 
Presenter
·         Thomas (Tom) Coleman, Senior, Psychology
Mentors
·         Sheri Mizumori, Psychology
·         Marsha Penner, Psychology
The hippocampus is well known for its role in spatial memory. A better understanding of how the hippocampus functions will provide us with insight into how memories are made and stored. This knowledge may ultimately lead to improved preventative and treatment strategies under conditions in which the hippocampus is altered, such as after traumatic brain injury or in cases of age-related memory impairment. Within the hippocampus there are cells commonly known as “place cells”. These cells are most likely to fire when a navigating organism is in a certain space in an environment, known as that cell’s “place field”. The activity of place cells may be modulated by dopamine input, which can be released tonically or phasically. For example, when overall dopamine input from the ventral tegmental area is disrupted, the place fields of cells within the CA1 subregion of the hippocampus are disrupted and spatial memory deficits occur. To test the idea that phasic dopamine, in particular, is necessary for normal place cell activity and spatial memory performance, we tested spatial working memory while recording electrophysiological activity of neurons in the hippocampus in normal mice and genetically engineered ‘knockout’ mice whose phasic dopamine signaling was selectively disrupted. Preliminary electrophysiological results show differences between the groups, including a nearly significant increase in the spatial selectivity that corresponds to a significant decrease in the size of place fields from the knockout mice when compared to controls. Behaviorally, knockout mice adapt more quickly than control mice following a change in the location of a reward, indicating that phasic dopamine input is not required to perform this task. Rather, removal of the phasic firing pattern may have unmasked a potential facilitating role of tonic dopamine release in flexible goal directed behaviors.

Thursday, May 17, 2012 - 4:43pm

Chantelle Kinzel will present her work during the poster presentation of the Mary Gates Undergraduate Research Symposium.  Chantelle's abstract is reprinted below.

 
 
Presenter
·         Chantelle Kinzel, Senior, Psychology
Mentor
·         Sheri Mizumori, Psychology
Ventral tegmental area (VTA) dopaminergic cell firing encodes reward information and has been implicated in reinforcement learning, spatial memory and decision-making. However the mechanisms which modulate VTA dopaminergic burst firing are not well known. A division of the mesonpontine tegmentum, the laterodorsal tegmental nucleus (LDTg) provides inputs into the VTA. Inactivation of the LDTg greatly reduces VTA dopaminergic burst firing, implicating the LDTg’s role in permitting VTA activity. In the current study we sought to determine the nature of the information coded by LDTg neurons in order to better understand the type of information that regulates dopamine cell activity. We assessed this by neurophysiological single unit recordings of LDTg cell activity in freely behaving rats. Rats were trained on an 8-arm radial maze, which required spatial and working memory in order to, without errors, forage for large and small rewards located at the end of arms. Experimental manipulations consisted of omitting expected rewards, switching the location of rewards of different magnitudes and imposing darkness during the maze trials. We recorded a total of 83 different cells, which were histologically confirmed to be located in the LDTg. We found a large number of reward-related cells (9.6% became excited when rats encountered reward, 8.4% showed excitation in anticipation of reward, 4.8% showed excitatory, then inhibitory and then excitatory responses around the time of reward) and movement related cells (76% were correlated with the rat’s velocity). The large proportion of velocity-correlated cells supports the LDTg’s role in motor behavior. Context and reward manipulations did not consistently affect the firing rate of LDTg cellular activity. However, the current study demonstrates that with 25% of LDTg cells exhibiting reward-related responses, the LDTg has a role in reward encoding by a permissive affect on VTA dopaminergic burst firing.

Thursday, May 17, 2012 - 4:40pm

Nile Graddis will present his work during the poster presentation of the Mary Gates Undergraduate Research Symposium.  Nile's abstract is reprinted below.

Phasic Dopamine Release Inhibits Hippocampal Gamma Oscillations

Presenter

·         Nile Graddis, Junior, Psychology Mary Gates Scholar

Mentors

·         Sheri Mizumori, Psychology

·         Marsha Penner, Psychology

Learning and memory require that neural activity be flexibly organized. Neurons must sometimes fire together in ensembles so that important signals are not lost in the complex activity of the brain, but these ensembles must also be capable of forming, breaking apart, or reforming at a moment’s notice. Such transient synchrony of neural activity may be achieved by means of oscillatory firing. Oscillatory firing at various frequencies is commonly found in electrophysiological recordings from the brain and may underlie the dynamic organization of neural activity. We investigated oscillatory activity in a brain region critical to learning and memory, the hippocampus. The power of oscillatory activity in the hippocampus at several frequency bands including gamma (30-80 hz) has been linked to successful encoding and recall in hippocampus-dependent memory tasks. Prior work has also shown that inputs from the ventral tegmental area are necessary for normal hippocampal function and for spatial learning and memory. These inputs occur in two forms: tonic baseline firing and intermittent phasic bursts. We tested genetic knockout mice that lack phasic but not tonic inputs from the ventral tegmental area while animals foraged for food on a spatial navigation task. We simultaneously recorded local field potentials in the hippocampus, which represent the net inputs to a region of the brain and allow us to assess the power of oscillatory activity. In the preliminary data, knockout mice demonstrated more rapid learning in response to successive manipulations of the reward location compared to controls. Knockouts also showed higher gamma-frequency but not theta-frequency (4-10 hz) power. These results suggest that phasic inputs from the ventral tegmental area selectively inhibit gamma-frequency oscillations in the hippocampus. This role may be behaviorally relevant, reflecting the possibility that these oscillations play a role in organizing learning and memory.


Thursday, May 17, 2012 - 4:36pm

Josh Larkin will present his data during the undergraduate oral seminars in the Behavioral and Neural Adaptation section of the Mary Gates Symposium.  Josh's abstract is reprinted below.

Neural and Behavioral Context-Based Differences in Rats

Presenter
·         Joshua (Josh) Larkin, Senior, Psychology Mary Gates Scholar
Mentors
·         Sheri Mizumori, Psychology
·         Marsha Penner, Psychology
Decision-making is a complex behavior where one must weigh cost and benefit to maximize the likelihood of the best possible outcome. A common example of this is a decision between a small yet certain reward (e.g. $1 or 1 piece of candy), and a larger uncertain reward (e.g. $10 or 10 pieces of candy 50% of the time). It has been suggested that context and dopamine levels play a critical role in the willingness of decision makers to take risks. We tested this hypothesis by assessing behavioral and neural differences between groups of rats trained on an equivalent risk-based paradigm conducted in different contexts. Rats started training in an operant chamber or a decision maze, and then switched contexts (i.e. tasks). These tasks involved the rat making a decision between two levers or doors, one of which was associated with a certain small reward (1 sugar pellet), and the other which is associated with a large (4 sugar pellets) reward that appeared with decreasing probability (100%, 50%, 25%, 12.5%). To test the influence that dopamine has on risk taking behavior, we used a dopamine antagonist (flupenthixol) to inhibit dopamine in both the maze and operant chamber contexts. Our results suggest that when analyzed as a group, rats are less willing to take risks in the maze context. We hypothesize this is because risk taking on the maze is a more complex behavior, perhaps because of a longer delay between a choice and reward delivery. Our pharmacological findings of high numbers of trials in which levers were not pressed in the chamber, or trials not run on the maze, suggest that blocking dopamine interferes with the ability of rats to make risky decisions. Together, these results support the idea that risk-based decision-making is influenced by many factors, including context and dopamine.

 


Friday, December 16, 2011 - 12:47pm

Joshua Larkin and Nile Graddis work in Sheri Mizumori's laboratory under the supervision of Marsha Penner (postdoc). Both Josh and Nile investigate (from different perspectives) interactions between the hippocampus and midbrain dopamine systems so that we can better understand the neural basis by which context information shapes reward-based learning.  

Nile's project is titled Finding lunch: The role of phasic dopamine release in the hippocampus

Josh's research is titled The effect of context and midbrain inactivation on risk task learning.


Thursday, May 26, 2011 - 11:58am

Jane Lee was awarded an Undergraduate Research Conference Travel Award from the Undergraduate Research Program to attend the Psychology Undergraduate Research Conference in Los Angeles, CA (May 6 2011).

 


Friday, May 13, 2011 - 1:42pm

The Mizumori lab has serveral recent articles published

 

Ventral tegmental area and substantia nigra neural correlates of spatial learning (2011). Adria K Martig and Sheri JY Mizumori. Learning and Memory, 18; 260-272.

 

Independent neural coding of reward and movement by pendunculopontine tegmental nucleus neurons in freely navigating rats. Alix BW Norton, Yong Sang Jo, Emily W Clark, Cortney A Taylor, and Sheri JY Mizumori. Eur J Neuroscience; Early View; 1-12.

 

Activation of dopamine neurons is critical for aversion conditioning and prevention of generalized anxiety. Larry S Zweifel, Jonathan P Fadok, Emmanuela Argilli, Micheal G Garelick, Graham L Jones, Tavis MK Dickson, James M Allen, Sheri JY Mizumori, Antonello Bonci and Richard D Palmiter. Nature Neuroscience, 14(5); 620-628.


Monday, April 11, 2011 - 4:35pm

Adaptive decisions during goal-directed navigation depend on a hierarchy of systems and cellular level interactions in the brain. This video demonstrates on a basic level, the relative involvement of the hippocampus, the dopamine system, and the ventral and dorsal (medial and lateral) striatum during a simple food search task on a laboratory maze. Particular attention is paid to the relative contributions of these brain areas while the rat initially learns about the environment, makes choices in a familiar environment, how decisions are adjusted when familiar conditions change.

 

[jwplayer|config=hdplayer|file=https://depts.washington.edu/mizulab/sites/default/files/pictures/rat_final.flv]

 


Wednesday, March 23, 2011 - 10:20am

Undergraduate research students in the Mizumori lab will be presenting their work during the 2011 UW Undergraduate Research Festival

 

  1. Jane Lee; The medial prefrontal cortex modulates anticipatory activity of dopaminergic neurons in the ventral tegmental area
  2. Tom Coleman; The role of phasic dopamine in the modulation of hippocampal place cell stability
  3. Josh Larkin; A Novel Maze-Based Task to Assess Decision Making in Rats

Wednesday, March 23, 2011 - 10:12am

Jane Lee will present her project titled “The Medial Prefrontal Cortex Modulates Anticipatory Activity of Dopaminergic Neurons in the Ventral Tegmental Area” at the 20th Annual UCLA Psychology Undergraduate Research Conference on May 6, 2011.